| Biological sciences & biotechnology |
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| Comparison of phenoloxidase isozymes from two color varieties of Tenebrio molitor L |
| HUANG Qiong1,2, HU Jie3, ZHOU Dinggang4, GOU Lin5 |
| 1. The Provincial Key Laboratory of Forest Protection, Sichuan Agricultural University, Ya′an, Sichuan 625014, China; 2. Forestry Ecological Engineering in the Upper Reaches of Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya′an, Sichuan 625014, China; 3. College of Economics and Management, Sichuan Agricultural University, Chengdu 611130, China; 4. College of Animal Science and Technology, Sichuan Agricultural University, Ya′an, Sichuan 625014, China; 5. Department of Biology, College of Life Science, Sichuan Agricultural University, Ya′an, Sichuan 625014, China |
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Abstract In order to provide some references for studying the bodycolor diversity, immune defence and variety selection of Tenebrio molitor, the phenoloxidase (including monophenol oxidase and diphenol oxidase) isozyme zymograms and activity at different developmental stages of the yellow and blackcolor varieties of T. molitor were compared using vertical polyacrylamide gel electrophoresis and spectrophotometry. The results showed that, during their developmental stages, six monophenol oxidase (MPO) bands and five diphenol oxidase (DPO) bands with different mobility were found, in which the MPO bands were distributed in three zones, while the DPO bands were in two zones. Meanwhile, the eggs of the blackcolor variety showed one more distinctive MPO bands compared with those of the yellowcolor variety. Moreover, the MPO and DPO activities of blackcolor variety eggs, larvae and adults were significantly higher than those of the yellowcolor ones. But at the pupal stage, the MPO activity of the yellowcolor variety was higher than that of the blackcolor one, while the DPO activity was opposite. Distinctive variations were shown in the band numbers, migration rate of enzyme bands and strength of enzymatic activity of MPO and DPO at different developmental stages of both color varieties. Compared with other developmental stages, there was one MPO and one DPO characteristic enzyme band at egg stage of both color varieties, but only one typical DPO band at larval stage. Meanwhile, there was different change on the zymograms and the activities of MPO and DPO of both color varieties at different developmental stages. From the egg to adult stage of both color varieties, the MPO activity changed in the descending order of egg stage ＞ pupal stage ＞ adult stage ＞ larval stage, while the DPO activity varied in the descending order of larval stage ＞ adult stage ＞ pupal stage ＞ egg stage. The results above lay a theoretical foundation for further research on the bodycolor diversity, immune defence and variety selection of T. molitor.
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Published: 25 July 2012
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2种色型黄粉虫酚氧化酶同工酶的比较
为黄粉虫(Tenebrio molitor L.)品种选育、体色分化及免疫防御研究提供参考，采用不连续聚丙烯酰胺凝胶垂直板电泳并结合分光光度法，对黄、黑2种色型黄粉虫各发育期的酚氧化酶（包括单酚氧化酶和二酚氧化酶）同工酶酶谱及酶活性进行比较研究。结果表明：在2种色型黄粉虫的各发育期，共发现迁移率不同的单酚氧化酶（MPO）同工酶酶带6条、二酚氧化酶（DPO）同工酶酶带5条，其中MPO酶带分布在3个泳动区，DPO酶带分布在2个泳动区，黑色型卵比黄色型卵多1条MPO特征性酶带；并且黑色型卵、幼虫和成虫的MPO和DPO活性显著高于黄色型卵、幼虫和成虫；但在蛹期，MPO活性黄色型比黑色型高，而DPO活性则是黑色型比黄色型高；此外，同一色型黄粉虫在不同发育阶段的MPO和DPO同工酶酶带数、酶活性强弱及迁移率变化明显，其中，卵期有MPO和DPO特有酶带各1条，幼虫期只有1条DPO特有酶带；同时，各发育阶段的MPO和DPO同工酶酶谱及酶活性变化规律不同，由卵至成虫期，MPO活性变化趋势为：卵期＞蛹期＞成虫期＞幼虫期，DPO活性变化趋势为：幼虫期＞成虫期＞蛹期＞卵期。上述结果为进一步研究黄粉虫的体色分化、免疫防御和品种选育奠定了理论基础。
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