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浙江大学学报（农业与生命科学版）
  2011, Vol. 37 Issue (6): 624-630    DOI: 10.3785/j.issn.1008-9209.2011.06.006
Biological sciences & biotechnology     
Study on astaxanthin accumulation by heterotrophic transformation in Haematococcus pluvialis
ZHANG Rui‐qin , GUAN Bin , KONG Qing , YOU Yong , WU Ji‐qin , WEI Qun
Institute o f Food Science and Engineering , Ocean University o f China , Qingdao , Shandong 266003 ,China
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Abstract  The effects of heterotrophic transformation and autotrophic transformation on astaxanthin production in H aematococcus p luv ialis were studied and compared . When sodium acetate was added in MCM for heterotrophic transformation ,astaxanthin accumulation was increased from ０ .７２３ ６ mg.L － １ to １.２１６ ８ mgL － １ .To enhance the astaxanthin accumulation , single factor experiment was founded up.Center composite design experiments and response surface methodology were employed to optimize the media of heterotrophic transformation.The results showed a distinctive influence of sodium acetate and potassium nitrate concentrations on astaxanthin accumulation.The optimal fermentation medium consisted of １.４６０ ５ g.L － １ sodium acetate , ０.００８ ４ g.L － １ potassium nitrate , and ０.０２ g.L － １ potassium dihydrogen phosphate.Astaxanthin content reached １０.９０８ mg .L － １ , which was raised １０.１６‐fold against the control group and １４.０７‐fold against the group of autotrophic transformation.

Published: 25 November 2011
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ZHANG Rui‐qin
GUAN Bin
KONG Qing
YOU Yong
WU Ji‐qin
WEI Qun
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ZHANG Rui‐qin,GUAN Bin,KONG Qing,YOU Yong,WU Ji‐qin,WEI Qun. Study on astaxanthin accumulation by heterotrophic transformation in Haematococcus pluvialis. , 2011, 37(6): 624-630.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.06.006     OR     http://www.zjujournals.com/agr/Y2011/V37/I6/624


雨生红球藻异养转化产虾青素的条件研究

对比自养转化与异养转化的虾青素产量,发现向培养基中添加乙酸钠进行异养转化可使羽生红球藻的虾青素产量由０.７２３ ６ mg.L － １ 提高到１.２１６ ８ mg.L － １ ;为进一步提高虾青素产量,在单因素实验基础上,选择乙酸钠浓度和硝酸钾浓度为自变量,虾青素产量为响应值,利用中心组合设计实验和响应面分析法,对转化培养基进行优化.结果表明:异养转化培养基中的乙酸钠和硝酸钾浓度对虾青素产量影响较大,在乙酸钠１.４６０ ５ g.L － １ ,硝酸钾０.００８ ４ g.L － １ ,磷酸二氢钾０.０２ g.L － １ 时,虾青素产量最
高,达１０.９０８ mg.L － １ ,与对照相比提高了１０.１６ 倍,与自养转化相比提高了１４.０７ 倍.
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