Development and application of a phosphate-dissolving bacteria agent
YE Ming,TAO Tao,CHEN Wu-xi
YE Ming,Schoolof Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China TAO Tao,Schoolof Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China CHEN Wu-xi,Schoolof Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009, China
Abstract Five bacterial strains with strong phosphate-solubilizing ability were mixed fermentation by C53 composite design,and the co-fermentation systems consisted of the strains Bc,Bs,Ap1 were obtained . The optimum inoculation in degradation of insoluble phosphate in soil was developed through orthogonal design,and the best inoculation ratio was Bc 2%,Bs 6%,Ap1 6% . It was a phosphate-dissolving bacteria agent that the three strains were inoculated in the phosphate-dissolving bacteria fermentation medium according to above-mentioned inoculation ratio and cultured at 30℃and shaking speed 140 r. min-1 for 28 h . Pot experiment results illustrated that the phosphate-solubilizing bacteria had a good advance on the plant height,root length and shoot fresh mass of Chinese cabbage .
YE Ming,TAO Tao,CHEN Wu-xi. Development and application of a phosphate-dissolving bacteria agent. Journal of Zhejiang University: Agric. & Life Sci., 2010, 36(6): 657-661.