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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2012, Vol. 38 Issue (6): 732-740    DOI: 10.3785/j.issn.1008-9209.2011.10.081
资源与环境科学     
  铜绿假单胞菌M2联合小白菜对Pb污染土壤的生物修复
  陈宝1，2， 徐晓萌2， 曲娟娟1*， 董蕾1， 闫立龙1， 孙兴滨3  
1.东北农业大学 资源与环境学院 寒地黑土资源利用与保护重点试验室，黑龙江 哈尔滨150030；2.浙江大学 环境与资源学院 污染环境修复与生态健康教育部重点试验室，浙江 杭州 310058；3.东北林业大学 林学院，黑龙江 哈尔滨150040
Bio-remediation of lead polluted soil by Pseudomonas aeruginosa strain M2 combined with pakchoi
CHEN Bao1,2, XU Xiao-meng2, QU Juan-juan1*, DONG Lei1, YAN Li-long1, SUN Xing-bin3
1. College of Resources and Environment College, Northeast Agricultural University, Harbin 150030, China; 2. Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China; 3. College of Forestry, Northeast Forestry University, Harbin 150040, China
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摘要： 从Pb污染矿区筛选到1株铜绿假单胞菌 (Pseudomonas aeruginosa) (M2)，采用盆栽试验研究其对Pb污染土壤中小白菜生长、Pb吸收和根际微生物的影响. 结果表明：接种M2菌后小白菜生物量提高20.9 %~31.3 %，地上部Pb含量提高15.0 %~25.4 %，根部Pb含量提高15.6 %~36.8 %；对可培养微生物数量的测定表明，在同样的Pb污染浓度下，接种铜绿假单胞菌的土壤中细菌和真菌数量明显提高，细菌提高17.8 %~29.6 %，真菌提高7.2 %~15.4 %，而放线菌提高不明显；细菌16S rDNA的变性梯度凝胶电泳分析表明，接种铜绿假单胞菌的土壤细菌种类比对照土壤多50.0 %~60.0 %. 结果证明，接种铜绿假单胞菌能够促进小白菜生长，维持土壤细菌多样性，对土壤Pb污染的植物修复有明显的促进作用.
Abstract:   As one of the largest mollisol area in the world, the black soil in northeast of China is commonly thought to be fertile and productive. But recently, dozens of blood heavy metal exceeding events were caused by heavy metal polluted agricultural production through food chain. Soil quality of some farmland near a mining site is getting worse and the content of heavy metal has already exceeded the third level of environmental quality standard for soil in China (GB15618-1995). Crops harvested from these areas had high level of heavy metal and brought a further health problem to human. In order to decrease the adverse effect of heavy metal accumulation in soil, the phytoremediation technology, which is efficient, costless and non-secondary pollution compared with traditional method, has become a hot research point. In many researches, it is a promising way to enhance heavy metal absorption from soil through increasing plants biomass, improving plants resistant to heavy metal and raising the concentration of heavy metal in plants. As many studies proved, inoculation of plant growth-promoting microbes can not only increase plant biomass and nutrition through a number of mechanisms such as nitrogen fixation, production of phytohormones and siderophores, and transformation of nutrient elements, but also can enhance tolerance and accumulation of metals in plants. This experiment was carried out to investigate the effect of bacterium application on the performance of pakchoi (Brassica rapa L. ssp. chinensis) and Pb removal from a contaminated soil. One Pseudomonas aeruginosa strain M2, originally isolated from mining area contaminated with Pb, was examined for its effect on the pakchoi growth, Pb accumulation and rhizospheric microorganism in soil spiked with Pb(NO3)2 in a pot experiment. The strain was cultivated in nutrient medium at 30 ℃ for 48 h, whose cells were collected in the exponential phase by centrifugation at 6 000 r/min for 10 min, and were inoculated to soil pretreated with Pb(NO3)2 with 108 CFU per gram fresh soil, and the non-inoculated pots were used as controls. The results showed that the biomass of pakchoi was increased by 20.9 % - 31.3 %, and the Pb concentrations in the above- and below-ground plant parts were increased by 15.0 % - 25.4 % and 15.6 % - 36.8 %, respectively, compared with the control pots of non-inoculated strain M2 (Table 1). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis showed that inoculation with strain M2 changed significantly the microbial community structures of the soils treated with Pb(NO3)2 of different concentration (Fig. 1). Moreover, cluster analysis and principal component analysis (PCA) indicated that the number of bacteria, fungi in inoculated soils increased by 17.8 % - 29.6 % and 7.2 % - 15.4 % respectively, however the number of actinomycetes was not significantly affected, as compared with the control under the same Pb(NO3)2 concentration (Fig. 2, 3). The DGGE analysis showed that the number of bacterial species in soil increased by 50.0 % - 60.0 % after inoculation with strain M2 (Fig. 1B). It can be concluded from the above findings that P. aeruginosa strain M2 combined with pakchoi not only can remediate Pb polluted soil but also improve the growth of pakchoi by sustaining microbial species diversity.
出版日期: 2012-11-20
基金资助:

环保公益性行业科研专项资助项目(2010467038).
通讯作者: 曲娟娟，E-mail: juanjuanqu@126.com   
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闫立龙1
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引用本文:

陈宝1,2， 徐晓萌2， 曲娟娟1*， 董蕾1， 闫立龙1， 孙兴滨3.   铜绿假单胞菌M2联合小白菜对Pb污染土壤的生物修复[J]. 浙江大学学报(农业与生命科学版), 2012, 38(6): 732-740.

CHEN Bao1,2, XU Xiao-meng2, QU Juan-juan1*, DONG Lei1, YAN Li-long1, SUN Xing-bin3. Bio-remediation of lead polluted soil by Pseudomonas aeruginosa strain M2 combined with pakchoi. , 2012, 38(6): 732-740.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.10.081    或    http://www.zjujournals.com/agr/CN/Y2012/V38/I6/732

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