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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2013, Vol. 39 Issue (5): 522-530    DOI: 10.3785/j.issn.1008-9209.2013.01.032
农业科学     
小菜蛾取食对2个芥菜变种芥子油苷组分和含量的影响
杨涛1, 石瑜1， 王晓艳2， 张新1， 钱红梅1， 孟秋峰3， 汪炳良1*
（1.浙江大学蔬菜研究所，杭州310029；2.温州市农业科学研究院蔬菜研究所，浙江 温州325006；
3.宁波市农业科学研究院蔬菜所，浙江 宁波315040）
Effect of diamondback month (Plutella xylostella) feeding on glucosinolate  profile and content change in two mustard (Brassica juncea) varieties
YANG Tao1, SHI Yu1, WANG Xiaoyan2, ZHANG Xin1, QIAN Hongmei1, MENG Qiufeng3, WANG Bingliang1*
(1.Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, China; 2.Institute of Vegetable Sciences, Wenzhou Academy of Agricultural Sciences, Wenzhou, Zhejiang 325006, China; 3. Institute of Vegetables, Ningbo Academy of Agricultural Sciences, Ningbo, Zhejiang 315040, China)
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摘要： 以2个芥菜变种（大叶芥和宽柄芥）为材料，研究小菜蛾对芥菜为害的选择性，并采用高效液相色谱法分析小菜蛾取食和机械损伤对叶片中芥子油苷组分和含量的影响。结果发现，小菜蛾对大叶芥的为害程度明显大于宽柄芥。在正常生长的宽柄芥和大叶芥叶片中分别检测到6种和8种芥子油苷，其中2-丙烯基芥子油苷是2个变种叶片中最主要的组分，占总含量的92%以上。宽柄芥叶片总芥子油苷含量极显著低于大叶芥，两者分别为7.21 μmol/g和13.31 μmol/g。当叶片被小菜蛾取食24 h后，2个变种总芥子油苷含量均明显升高，且宽柄芥叶片升高的幅度远大于大叶芥，分别达21.35 μmol/g和20.49 μmol/g；而2个变种各组分中2-丙烯基芥子油苷含量升高更明显，且宽柄芥叶片升高的幅度同样远大于大叶芥。同时，小菜蛾取食诱导2个变种叶片合成了新的芥子油苷组分，其中大叶芥叶产生了一种含量很低的吲哚族芥子油苷（4-羟基吲哚-3-甲基芥子油苷），而宽柄芥中产生了一种低含量的脂肪族芥子油苷（10-甲基亚磺酰癸基芥子油苷）和一种低含量的吲哚族芥子油苷（1-甲氧吲哚-3-甲基芥子油苷）。研究表明，2个芥菜变种中叶片芥子油苷含量高者受小菜蛾为害重，小菜蛾取食会诱导芥菜产生新的芥子油苷，并且诱导芥子油苷大量合成，但对不同变种的诱导作用存在差异。2-丙烯基芥子油苷在芥菜响应昆虫取食胁迫中发挥主要作用，且受害程度轻的芥菜中2-丙烯基芥子油苷对昆虫取食胁迫的反应更敏感。  
Abstract: Mustard (Brassica juncea Coss.) is an original Chinese vegetable with agricultural importance in brassica family of  Cruciferae. There are about 16 varieties, including B. juncea var.  rugosa, B. juncea var. latipa and B. juncea var. tumida, and so on. They  distribute widely in Sichuan and Zhejiang in China. Mustard is often attacked by herbivorous insects (e.g. diamondback month, Plutella xylostella) during different development stages. Glucosinolates (GSs) are believed to have multiple functions in plant-pest interactions, providing not only feeding and ovipositioning stimulants but also compounds acting as toxins and feeding deterrents to generalist insect herbivores. However, little was known about the effect of diamondback month feeding on glucosinolate profile and content in mustard. In the present study, in order to investigate the effect of GSs on plant resistance to herbivores, the larval preference of diamondback moth was studied in two mustard varieties, B. juncea var. rugosa and B. juncea var. latipa, and the changes in glucosinolate profile and content were analyzed by high performance liquid chromatography (HPLC) in diamondback moth fed leaves and mechanical wounded leaves. The uniform and healthy seedlings with 45 true leaves from the two varieties were selected and 
divided into two groups. One group, including the seedlings from each of the different varieties were  placed in a randomly assigned position in the same diagonal grid within the cage. Six fourth instar larvae which were newly emerged and had been starved for four  hours were placed in the centre of cage. The area of the damaged leaves and the number of larvae on each plant were recorded 24 hours after treatments. The seedlings of other group were  cut by 1/3 of the whole leaf with scissors as mechanical wounding, and the samples were harvested 24 hours after treatment. Prior to the larvae feeding and mechanical wounding, 1520 g of the untreated samples were taken from the seedlings of each variety as  control, and all of the samples were  harvested, freeze-dried and analyzed for GSs. The results showed that there was significantly heavier injury in B. juncea var. rugosa than in B. juncea var. latipa. Six and eight GSs were detected in healthy leaves of B. juncea var. latipa and B. juncea  var. rugosa, respectively, and 2-propenyl GS was the major profile in two varieties, accounting for more than 92% of the total GSs content. Significantly lower total GS content was observed in healthy leaves of B. juncea var. latipa (7.21 μmol/g) than that in B. juncea var. rugosa (13.31 μmol/g). The content of total GSs in two varieties increased significantly after attracted by diamondback moth for 24 h, and the increasing extent was significantly higher in B. juncea var. latipa  than in B. juncea var. rugosa, reaching 20.49 μmol/g  and 21.35 μmol/g, respectively. Furthermore, the highest increase was observed in the content of 2-propenyl GS in both two varieties, and the increasing extent was also significantly higher in B. juncea var. latipa than in B. juncea var. rugosa. In addition, several glucosinolate profiles were induced after larvae feeding, an indole GS (4-hydroxyindol-3-ylmethyl GS) was induced in B. juncea var. rugosa at low level, while an aliphatic GS (10-methylsulfinyldecyl GS) and an indole GS (1-methoxy-3-indolymethyl GS) with low level were newly detected in B. juncea var. latipa. Likewise, the content of total GSs, as well as 2-propenyl GS increased significantly at similar rate between two varieties tested after mechanical wounding treatment. Furthermore, 10-methylsulfinyldecyl GS was also detected in B. juncea var. latipa at low level after mechanical wounding treatment, but 1-methoxy-3-indolymethyl GS was not detected. 4-hydroxyindol-3-ylmethyl GS was not identified in B. juncea var. rugosa. In conclusion, our results indicate  that the mustard variety with higher GS content is more susceptible to diamondback moth. Both diamondback moth feeding and mechanical wounding could induce the biosynthesis of glucosinolates with difference in glucosinolate profile and increasing rate. It is also suggested  that 2-propenyl GS might play an important role in plant-environment interactions, and 2-propenyl GS in the resistant mustard variety seems to be more susceptible to the feeding of herbivorous insects.
出版日期: 2013-09-20
CLC:  S 637  
基金资助:

科技部农业成果转化资金项目（2012GB2C200184;2011GB2C220003）；浙江省重点科技创新团队建设项目（N20110277）；
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引用本文:

杨涛1, 石瑜1， 王晓艳2， 张新1， 钱红梅1， 孟秋峰3， 汪炳良1*. 小菜蛾取食对2个芥菜变种芥子油苷组分和含量的影响[J]. 浙江大学学报(农业与生命科学版), 2013, 39(5): 522-530.

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http://www.zjujournals.com/agr/CN/Y2013/V39/I5/522

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