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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2009, Vol. 10 Issue (3): 188-192    DOI: 10.1631/jzus.B0820240
Biomedicine     
Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells
Zhi YANG, Miao LIU, Yin-gang ZHANG, Xiong GUO, Peng XU
Department of Orthopaedics, the First Affiliated Hospital, School of Medicine; MOE Key Laboratory of Environment and Genes Related to Diseases, Institute of Environmental and Endemic Diseases; School of Materials Science and Engineering, Xi’an Jiaotong University, Xi’an 710061, China
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Abstract  Objective: We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells (BMSCs) in vitro. Methods: BMSCs were isolated from adult marrow donated by a hip osteoarthritis patient with prosthetic replacement and cultured in vitro. The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently (pressure: 50 kPa, 30 min/times, and twice daily). The control was cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast microscopy, the determination of alkaline phosphatase (ALP) activities, and the immunohistochemistry of collagen type I. The mRNA expressions of osteoprotegerin (OPG) and osteoprotegerin ligand (OPGL) in BMSCs were analyzed by real-time polymerase chain reaction (PCR). Results: BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure, the activity of ALP increased significantly, and the expression of collagen type I was positive. In the treatment group, the mRNA expression of OPG increased significantly (P<0.05) and the mRNA expression of OPGL decreased significantly (P<0.05) after 2 weeks, compared with the control. Conclusion: Intermittent negative pressure could promote osteogenesis in human BMSCs in vitro.

Key words： Negative pressure      Bone marrow-derived stroma cells (BMSCs)      Osteogenesis     
Received: 07 August 2008     
CLC:  R687  
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Zhi YANG
Miao LIU
Yin-gang ZHANG
Xiong GUO
Peng XU
Cite this article:

Zhi YANG, Miao LIU, Yin-gang ZHANG, Xiong GUO, Peng XU. Effects of intermittent negative pressure on osteogenesis in human bone marrow-derived stroma cells. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(3): 188-192.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820240     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2009/V10/I3/188

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