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Waste Disposal & Sustainable Energy
Waste Disposal & Sustainable Energy  2019, Vol. 1 Issue (3): 199-206    DOI: 10.1007/s42768-019-00014-y
    
二乙酸荧光素和碘化丙啶荧光染色在监测藻类生长和生存力中的适用性
Swati Yewalkar1, Tong Wu1, David Kuan1, Heli Wang2, Di Li2, Andy Johnson3, Dusko Posarac1, Sheldon Duf1, Xiaotao T. Bi1
1 Department of Chemical and Biological Engineering, The University of British Columbia, Vancouver, Canada  2 School of Water Resource and Environment, China University of Geosciences, Beijing, China  3 Biomedical Research Center, The University of British Columbia, Vancouver, Canada
Applicability of differential fluorescein diacetate and propidium iodide fluorescence staining for monitoring algal growth and viability
Swati Yewalkar1, Tong Wu1, David Kuan1, Heli Wang2, Di Li2, Andy Johnson3, Dusko Posarac1, Sheldon Duf1, Xiaotao T. Bi1
1 Department of Chemical and Biological Engineering, The University of British Columbia, Vancouver, Canada  2 School of Water Resource and Environment, China University of Geosciences, Beijing, China  3 Biomedical Research Center, The University of British Columbia, Vancouver, Canada
 全文: PDF 
摘要： 可以培育微藻，通过生产酶来生产高价值的产品，以抵消二氧化碳封存的成本，从而提供财政激励。需要监测光生物反应器中藻类的生存能力，以确保具有生物活性的活细胞。本研究探索了一种简便的荧光检测法，采用二乙酸荧光素（FDA）和碘化丙丙啶（PI）荧光染色，用于区分光生物反应器中活藻细胞和死藻细胞。FDA给活细胞染上荧光绿，而PI给死细胞染上荧光绿，这样可以区分活细胞和死细胞。用在摇瓶和连续搅拌的光生物反应器中生长的两种绿藻和两种蓝藻对此方法进行了评估。该方法适用于蛋白核小球藻和7002多聚球藻，但不适用于双晶型和7942长链型多聚球藻的培养。我们得出结论，FDA是监测光生物反应器中活藻细胞的良好染色剂，但必须评估其对个别藻类物种的适用性。
关键词： 二乙酸荧光素;  碘化丙丙啶;  活藻和死藻;  差异染色;  连续光生物反应器     
Abstract: Microalgae can be cultivated for producing high-valued products through the production of enzymes to offset the cost of CO2 sequestration, providing financial incentives. The viability of algae in the photobioreactor needs to be monitored to ensure biologically active live cells. In this study, we explored a simple fluorometry method for differentiation of live and dead algal cells in photobioreactors by fluorescein diacetate (FDA) and propidium iodide (PI) fluorescence staining. FDA stains fluorescent green to the living cells while PI stains the dead cells, allowing the discrimination of live and dead cells. The method was evaluated using two green algae and two strains of cyanobacteria grown in shake flasks and a continuously stirred photobioreactor. The method was found applicable for Chlorella pyrenoidosa and Synechococcus 7002 but was not applicable for the cultures of Scenedesmus dimorphus and Synechococcus elongatus 7942. We conclude that FDA is a good stain for monitoring live algal cells in photobioreactors but its applicability to individual species of algae must be evaluated.
Key words: Fluorescein diacetate (FDA)    Propidium iodide (PI)    Live and dead algae    Diferential staining    Continuous    photobioreactor
收稿日期: 2019-07-30 出版日期: 2020-01-14
通讯作者: Xiaotao T. Bi     E-mail: xbi@chbe.ubc.ca
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Swati Yewalkar
Tong Wu
David Kuan
Heli Wang
Di Li
Andy Johnson
Dusko Posarac
Sheldon Duf
Xiaotao T. Bi

引用本文:

Swati Yewalkar, Tong Wu, David Kuan, Heli Wang, Di Li, Andy Johnson, Dusko Posarac, Sheldon Duf, Xiaotao T. Bi. Applicability of differential fluorescein diacetate and propidium iodide fluorescence staining for monitoring algal growth and viability. Waste Disposal & Sustainable Energy, 2019, 1(3): 199-206.

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http://www.zjujournals.com/wdse/CN/10.1007/s42768-019-00014-y    或    http://www.zjujournals.com/wdse/CN/Y2019/V1/I3/199

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