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浙江大学学报（理学版）
Journal of Zhejiang University (Science Edition)  2023, Vol. 50 Issue (1): 69-82    DOI: 10.3785/j.issn.1008-9497.2023.01.011
Chemistry     
Progress of photobiological hydrogen production by green algae
Yaqin ZHANG1,2(),Ruikang TANG1,2,Weimin MA3,Wei XIONG4,Xurong XU2()
1.Department of Chemistry，Zhejiang University，Hangzhou 310027，China
2.Qiushi Academy for Advanced Studies，Zhejiang University，Hangzhou 310027，China
3.College of Life Sciences，Shanghai Normal University，Shanghai 200234，China
4.School of Chemistry and Chemical Engineering，Nanchang University，Nanchang 330031，China
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Abstract  

Photobiological hydrogen production by green algae exhibits a bright application prospect in solar energy utilization and hydrogen energy production due to the advantages of high energy conversion efficiency, environmental friendliness as well as abundant raw materials. This paper analyzes the potential factors limiting photobiological hydrogen production by green algae based on the mechanism, and summarizes various methods to improve the efficiency of photobiological hydrogen production by green algae. The main problems and development trends in the commercial application of photobiological hydrogen production by green algae are briefly reviewed, which are referable for the large-scale application of photobiological hydrogen production by green algae in the future.

Key words： green algae      photobiological hydrogen      hydrogenase      O2 sensitivity      electronic sources     
Received: 30 March 2022      Published: 13 January 2023
CLC:  Q 949  
Corresponding Authors: Xurong XU     E-mail: 11837050@zju.edu.cn;xrxu@zju.edu.cn
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Yaqin ZHANG
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Cite this article:

Yaqin ZHANG,Ruikang TANG,Weimin MA,Wei XIONG,Xurong XU. Progress of photobiological hydrogen production by green algae. Journal of Zhejiang University (Science Edition), 2023, 50(1): 69-82.

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https://www.zjujournals.com/sci/EN/Y2023/V50/I1/69


绿藻光合产氢的研究进展

绿藻光合产氢具有能量转化效率高、环境友好、原料丰富等优势，在太阳能利用和氢能生产方面具有光明的应用前景。从绿藻光合产氢的生物学机理出发，分析了限制绿藻光合产氢的潜在因素，总结了各类提升绿藻光合产氢效率的方法，并简要评述了绿藻光合产氢实现商业化应用所面临的主要问题及发展趋势，为未来绿藻光合产氢的大规模应用提供参考。


关键词： 绿藻,  光合产氢,  氢化酶,  氧敏感性,  电子源 
Fig.1 Schematic representation of the H2 production pathway in green alga［12］
Fig.2 Model scheme for reaction of CO and O2 with oxidation state of the H-cluster［22］
产氢方法绿藻种类产氢条件产氢总量/（mL·L-1）产氢持续时间/d参考文献
氮气吹扫法C. reinhardtii氮气吹扫，补充二氧化碳<2060［31］
添加除氧剂C. reinhardtii CC50313 mol·L-1 NaHSO3，逐步添加法~1123［38］
C. reinhardtii葡萄糖氧化酶组合、氢氧化镁77826［39］
藻菌共培养Chlorella sp. MACC 360与大肠杆菌共培养~911［40］
控制光照法C. reinhardtii CC124脉冲光照条件（1 s光照/9 s黑暗）~732.25［41］
细胞聚集法ChlorellaPDADMAC诱导矿化172［42］
代谢调控法Chlorella protothecoidesTAP-S（0.35 mM NH4Cl）2344.17［43］
Chlorella sorokiniana strain CeTAP-S~1507.38［44］
基因突变法C. reinhardtii 突变体stm6TAP-S54014［45］
C. reinhardtii 质子梯度突变体pgr5TAP-S8509［46］
C. reinhardtii D1蛋白突变体L159I-N230YTAP-S50412［47］
基因改造法C. reinhardtii CC849基因改造hemHc-lbacTAP-S、氮气吹扫82.55［48］
C. reinhardtii 基因改造hydA-SOD融合酶葡萄糖氧化酶组合18414［49］
Table 1 Hydrogen production of various methods
Fig.3 Photobiological H2 production with C. pyrenoidosa by construction of a laccase-modulated anaerobic layer［60］
Fig.4 Schematic of photobiological and fermentative H2 production with the algae-bacteria cocultures （Chlamydomonas and E. coli cocultures）［61］
Fig.5 Spatial differentiation of chlorella aggregates for hydrogen production［42］
Fig.6 Schematics of the intracellular processes and pathways that occur under normal， sulfur-replete conditions and during sulfur-deprivation［71］
Fig.7 Summary of methods to improve photobiological hydrogen productionThe more the number of " + "， the higher the potential of improving hydrogen production efficiency.
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