Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L

doi:10.3785/j.issn.1008-9497.2014.04.015

Journal of ZheJIang University(Science Edition)

2014, Vol. 41

Issue (4): 435-439 DOI: 10.3785/j.issn.1008-9497.2014.04.015

Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L

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Received: 25 March 2013 Published: 01 April 2014

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	Articles by authors
	LIU Shengcai
	KUANG Huaqin
	XIE Liyang
	LAI Zhongxiong

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LIU Shengcai, KUANG Huaqin, XIE Liyang, LAI Zhongxiong. Cloning of miR319b precursor gene and expressing analysis during the in vitro plantlets development by qPCR in Amaranthus tricolor L. Journal of ZheJIang University(Science Edition), 2014, 41(4): 435-439.

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https://www.zjujournals.com/sci/10.3785/j.issn.1008-9497.2014.04.015 OR https://www.zjujournals.com/sci/Y2014/V41/I4/435

苋菜试管苗ama miR319b前体的克隆及其在苋菜试管苗发育过程中的表达分析

试验以苋菜试管苗及该试管苗发育过程中各阶段培养物为材料，进行miR319b前体的克隆及其在苋菜试管苗发育过程中的qPCR分析. 结果表明：采用RTPCR法克隆到苋菜miR319b前体序列，长186 bp.该前体与烟草、苜蓿等miR319b前体序列具有较高的同源性，并包含长21 bp的miR319b的成熟序列；利用qPCR技术研究显示，该miRNA在苋菜试管苗发育过程中的表达具有特异性，在幼苗期时相对表达量最低，而在壮苗期和花芽期相对表达量迅速增加，且在花芽期时达到最高值；进入开花期，其相对表达量又呈下降趋势，但略高于壮苗期. 说明amamiR319b前体在苋菜幼苗期向壮苗期转变及花芽期向开花期转变阶段可能起重要作用，这为将来进一步研究其功能奠定了基础.

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