Please wait a minute...
浙江大学学报（理学版）
浙江大学学报（理学版）  2016, Vol. 43 Issue (4): 436-441    DOI: 10.3785/j.issn.1008-9497.2016.04.010
生命科学     
不同温度下苏云金芽孢杆菌对金银花尺蠖幼虫的致病力
向玉勇, 隋姗姗, 刘冲, 郑怡
滁州学院 生物与食品工程学院, 安徽 滁州 239000
Pathogenicity of Bacillus thuringiensis to Heterolocha jinyinhuaphaga Chu larvae at different temperatures
XIANG Yuyong, SUI Shanshan, LIU Chong, ZHENG Yi
School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, Anhui Province, China
 全文: PDF(753 KB)  
摘要： 为了掌握苏云金芽孢杆菌（Bacillus thuringiensis）对金银花尺蠖（Heterolocha jinyinhuaphaga Chu）幼虫致病的最佳条件，测定了不同温度、不同浓度下的苏云金芽孢杆菌对金银花尺蠖幼虫的致死作用.结果表明，在15~35 ℃，苏云金芽孢杆菌浓度为1×104，1×105，1×106，1×107，1×108，1×109芽孢·mL-1，各浓度下对金银花尺蠖幼虫均有一定的致死作用，在31 ℃时校正死亡率达到最大，分别为60.32%，67.24%，72.41%，79.31%，84.48%和91.38%，同一温度下的校正死亡率随着浓度的升高显著上升.方差分析显示，温度和浓度的交互作用对金银花尺蠖幼虫的校正死亡率没有显著影响（p>0.05）.由所建立的拟合模型计算可得各浓度对应的校正死亡率最大温度分别为29.88，31.54，32.50，31.17，33.28和32.75℃.毒性分析表明，31 ℃时苏云金芽孢杆菌的毒性最强，LC50值为2.70×103芽孢·mL-1，高于或低于31℃，其毒性有所下降.
关键词： 苏云金芽孢杆菌;  金银花尺蠖;  温度;  校正死亡率;  毒性    
Abstract: To obtain the optimal pathogenicity conditions of Bacillus thuringiensis (Bt) to Heterolocha jinyinhuaphaga Chu larvae, the lethal effects of Bt on H. jinyinhuaphaga Chu larvae at different temperatures and dosages were tested in laboratory. The dosages of Bt were set as 1×104, 1×105, 1×106, 1×107, 1×108, 1×109 spores·mL-1. The result showed that Bt at each dosage had a certain lethal effect on H. jinyinhuaphaga Chu larvae in the range from 15 to 35 ℃. The corrected mortality reached the highest level at 31 ℃, which was 60.32%, 67.24%, 72.41%, 79.31%, 84.48% and 91.38%, separately, and it would decrease significantly at lower or higher temperatures. At the same temperature, the corrected mortality would increase when the dosage was added. Two way ANOVA showed that the interaction of temperatures and dosages had no significant effect on the corrected mortality of H. jinyinhuaphaga Chu larvae(p>0.05). According to the established regression model, the optimum temperatures for the biggest corrected mortality at each dosage were calculated as 29.88, 31.54, 32.50, 31.17, 33.28 and 32.75 ℃, respectively. Toxicity analysis showed that the toxicity of Bt was strongest at 31 ℃, and it's LC50 value was 2.70×103 spores·mL-1. The toxicity of Bt would decrease at the temperatures higher or lower than 31℃.
Key words: Bacillus thuringiensis    Heterolocha jinyinhuaphaga Chu    temperature    corrected mortality    toxicity
收稿日期: 2015-09-23 出版日期: 2016-04-28
CLC:  Q965  
基金资助: 安徽高校省级自然科学研究项目（KJ2012B123）；安徽省高等学校优秀青年人才基金（2009SQRZ147）；国家级大学生创新创业训练计划项目（201310377018）；安徽省高校优秀中青年骨干人才国内外访学研修重点项目（gxfxZD2016249）.
作者简介: 向玉勇(1974-),ORCID:http://orcid.org/0000-0001-9335-2256,男,博士,副教授,主要从事昆虫化学生态学及害虫防治研究,E-mail:xyy10657@sohu.com.
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
向玉勇
隋姗姗
刘冲
郑怡

引用本文:

向玉勇, 隋姗姗, 刘冲, 郑怡. 不同温度下苏云金芽孢杆菌对金银花尺蠖幼虫的致病力[J]. 浙江大学学报（理学版）, 2016, 43(4): 436-441.

XIANG Yuyong, SUI Shanshan, LIU Chong, ZHENG Yi. Pathogenicity of Bacillus thuringiensis to Heterolocha jinyinhuaphaga Chu larvae at different temperatures. Journal of ZheJIang University(Science Edition), 2016, 43(4): 436-441.

链接本文:

https://www.zjujournals.com/sci/CN/10.3785/j.issn.1008-9497.2016.04.010    或    https://www.zjujournals.com/sci/CN/Y2016/V43/I4/436

[1] 张文冉,高殿滑,刘爱华.金银花尺蠖的发生与气象条件的关系[J].气象与环境科学,2007,30(4):60-62. ZHANG Wenran, GAO Dianhua, LIU Aihua. Relationships between Honeysuckle Geometrid occurrence and meteorological condition[J]. Meteorological and Environmental Sciences,2007,30(4):60-62.
[2] 王广军,张国彦,王江蓉.金银花尺蠖的发生规律与防治技术[J].中国植保导刊,2005,25(3):22-23. WANG Guangjun, ZHANG Guoyan, WANG Jiangrong. Occuring laws of Heterolocha jinyinhuaphaga Chu and the control metholds[J]. China Plant Protection,2005,25(3):22-23.
[3] 姜敏,邵明果,赵伯林.金银花尺蠖的生物学特性及防治技术[J].山东林业科技,2005(1):62-63. JIANG Min, SHAO Minguo, ZHAO Bolin. Biology characteristic of Heterolocha jinyinhuaphaga Chu and the control metholds[J]. Shandong Forestry Science and Technology,2005(1):62-63.
[4] 向玉勇,刘克忠,殷培峰,等.安徽金银花尺蠖的生物学特性[J].滁州学院学报,2010,12(5):35-37. XIANG Yuyong, LIU Kezhong, YIN Peifeng, et al. The biological characteristics of Honeysuckle Geometrid in Anhui Province[J]. Journal of Chuzhou University,2010,12(5):35-37.
[5] 倪云霞,刘新涛,刘玉霞,等.金银花尺蠖的药剂防治[J].河南农业科学,2006(12):78-79. NI Yunxia, LIU Xintao, LIU Yuxia, et al. Pesticide control to Heterolocha jinyinhuaphaga Chu[J]. Journal of Henan Agricultural Sciences,2006(12):78-79.
[6] HAIDER M Z, ELLAR D J. Mechanism of action of Bacillus thuringiensis insecticidalδ-endotoxins: Interaction with phospholipid vesicles [J]. Biochimica et Biophysica Acta,1989,978:216-222.
[7] VAN RJ, JANSENS S, HÖFTE H, et al. Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins[J]. Applied and Environment Microbiology,1990,56(5):1378-1385.
[8] 戴莲韵,王学聘.苏云金芽孢杆菌研究进展[M].北京:科学出版社,1997. DAI Lianyun, WANG Xuepin. Study Progress on Bacillus Thuringiensis[M]. Beijing: Science Press,1997.
[9] WHALON M E,WINGERD B A. Bt: Mode of action and use[J]. Archives of Insect Biochemistry and Physiology,2003,54(4):200-211.
[10] 李平.苏云金杆菌新菌株EG4961对黄瓜十一星叶甲和马铃薯甲虫的幼虫和成虫的杀虫活性[J].农药译丛,1994,16(1):40-43. LI Ping. Insecticidal activity of Bacillus thuringiensis EG4961 on the larvae and adult of diabrotica undecimpunctata and Leptinotarsa decemlineata[J]. Pesticide Translations,1994,16(1):40-43.
[11] 竺莉红,桑金隆,李孝辉,等.苏云金芽孢杆菌Ba9808对鞘翅目害虫的生物活性[J].浙江农业学报,2002,14(6):331-333. ZHU Lihong, SANG Jinlong, LI Xiaohui, et al. Toxicity of Bacillus thuringiensis Ba9808 against the larvae of Coleoptera[J]. Acta Agriculturae Zhejiangensis,2002,14(6):331-333.
[12] 蔡鸿娇,侯有明,尤民生.应用Bt控制鞘翅目害虫的研究现状[J].武夷科学,2002,18:259-260. CAI Hongjiao, HOU Youmin, YOU Minsheng. Study status on the application of Bacillus thuringiensis against the pest of Coleoptera[J]. Wuyi Science Journal,2002,18:259-260.
[13] 姚江.苏云金芽孢杆菌及其在害虫防治上的应用[J].连云港师范高等专科学校学报,2002(1):36-41. YAO Jiang. Bacilus Thuringiensis and its application in prevention of insects[J]. Journal of Lianyungang Teachers College,2002(1):36-41.
[14] 汪敏捷,雷玲,王建华,等.不同温湿度下白僵菌对红缘天牛幼虫致病力的研究[J].天津师范大学学报:自然科学版,2013,33(3):79-82. WANG Minjie, LEI Ling, WANG Jianhua, et al. Pathogenicity of beauveria bassiana to Asias halodendri larvae under different temperatures and humidities[J]. Journal of Tianjin Normal University: Natural Science Edition,2013,33(3):79-82.
[1] 陈嘉星,江迪,张霄宇. 基于动态模态分解的长江口海表温度时空分布特征重构研究[J]. 浙江大学学报（理学版）, 2022, 49(1): 76-84.
[2] 倪群丽， 梁 兰， 徐 鑫， 林 琦. 金霉素对小麦根生长的毒性效应[J]. 浙江大学学报（理学版）, 2015, 42(6): 739-744.
[3] 向玉勇， 张 帆， 郑成钢. 扫描电镜下的金银花尺蠖触角感受器[J]. 浙江大学学报（理学版）, 2015, 42(6): 749-754.
[4] 夏阿根,徐芸,章林溪. 聚甲醛单链的玻璃化转变研究[J]. 浙江大学学报（理学版）, 2000, 27(6): 601-604.
[5] 于　丽 1,林瑞森 1,刘绪良 2,张洪林 3,孙海涛 3. 微量量热法研究温度对两种石油菌生长的影响[J]. 浙江大学学报（理学版）, 2000, 27(6): 628-632.
[6] 马海珠. 无规聚甲基丙烯酸甲酯链的温度系数[J]. 浙江大学学报（理学版）, 1999, 26(4): 64-66.