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工程设计学报
工程设计学报  2018, Vol. 25 Issue (4): 383-387,419    DOI: 10.3785/j.issn.1006-754X.2018.04.003
设计理论与方法学     
流式细胞仪样品流速及聚焦流测量方法研究
严心涛, 马玉婷, 吴云良, 武晓东, 王策
中国科学院 苏州生物医学工程技术研究所, 江苏 苏州 215163
Research on measurement method for sample flow rate and focusing core flow of flow cytometer
YAN Xin-tao, MA Yu-ting, WU Yun-liang, WU Xiao-dong, WANG Ce
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
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摘要：

为提高流式细胞仪的探测分辨率和数据检测的稳定性，需要精确控制样品流速，并分析样品流速和鞘液流速对样品聚焦流的影响，可通过样品聚焦流直径和样品聚焦流在流动室流道中的相对位置来评价样品的聚焦状况。利用蠕动泵运动特点，设计了一种平均流量称重法测量样品流速的方法，并与微流量传感器测量结果作比较；采用最小二乘法线性拟合蠕动泵的控制电压和样品流速之间的函数关系，并采用显微成像法直接测量和分析样品流速和鞘液流速对样品聚焦流直径、偏离流动室流道中心线的距离的影响。实验结果显示，采用平均流量称重法与微流量传感器测得的样品流速的线性相关系数高达0.982 8；蠕动泵的样品流速与其控制电压的线性相关系数高于0.99，说明利用该线性关系可以实现样品流速的精确控制；采用的显微成像法能快速方便地测得样品聚焦流的直径及位置，为流式细胞仪样品流速、鞘液流速的调控以及液流器件组装精度的测试提供了指导方法。
关键词： 流式细胞术;  流速;  蠕动泵;  流动室;  聚焦    
Abstract:

In order to improve the detection resolution and the stability of data detection, it is usually necessary to precisely control the sample flow rate in the flow system and analyze the effects of the sample flow rate and sheath fluid flow rate on the sample focusing core flow. The focusing state can be evaluated by the sample focusing core flow diameter and the relative position of the sample focusing core flow in the flow channel. First of all, according to the movement characteristics of peristaltic pump, an average flow weighing method was designed to measure the sample flow rate and compared with the measurement result of micro-flow sensor. Then, the relationship between the control voltage of the peristaltic pump and sample flow rate was linearly fitted by the least squares method. Lastly, the microscopic imaging method was used to measure and analyze the impact of the sample flow rate and sheath fluid flow rate on the diameter of the sample focusing core flow and its deviation distance from the flow channel centerline. The experimental data showed that the linear correlation coefficient of sample flow rate measured by the average flow weighing method and the micro-flow sensor was as high as 0.982 8. The correlation coefficient between the sample flow rate of the peristaltic pump and its control voltage was as high as 0.99, which showed that the linear relationship could be used to control the sample flow rate accurately. The diameter and position of sample focusing core flow were quickly and conveniently measured by the microscopic imaging method, which provided a guidance method for the regulation of the flow rate of samples and the sheath fluid in flow cytometer, and the test of the assembly accuracy of the flow device.
Key words: flow cytometry    flow rate    peristaltic pump    flow channel    focusing
收稿日期: 2017-10-10 出版日期: 2018-08-28
CLC:  TH789  
基金资助:

苏州市科技局民生科技计划资助项目（SS201726）；苏州市医疗器械与新医药专项（ZXY201428）
作者简介: 严心涛(1987-),男,湖北仙桃人,助理研究员,硕士,从事机电一体化、液流控制研究,E-mail:yanxt@sibet.ac.cn,https://orcid.org/0000-0002-2523-5063
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引用本文:

严心涛, 马玉婷, 吴云良, 武晓东, 王策. 流式细胞仪样品流速及聚焦流测量方法研究[J]. 工程设计学报, 2018, 25(4): 383-387,419.

YAN Xin-tao, MA Yu-ting, WU Yun-liang, WU Xiao-dong, WANG Ce. Research on measurement method for sample flow rate and focusing core flow of flow cytometer[J]. Chinese Journal of Engineering Design, 2018, 25(4): 383-387,419.

链接本文:

https://www.zjujournals.com/gcsjxb/CN/10.3785/j.issn.1006-754X.2018.04.003    或    https://www.zjujournals.com/gcsjxb/CN/Y2018/V25/I4/383

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