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Food Qual Safet  2019, Vol. 3 Issue (4): 265-272    DOI: 10.1093/fqsafe/fyz026
Research Articles     
γ射线辐照结合4℃贮藏对Legon-18辣椒粉中单核细胞增生李斯特菌、大肠杆菌和沙门氏菌灭活效果的影响
Effect of gamma radiation and storage at 4°C on the inactivation of Listeria monocytogenes, Escherichia coli and Salmonella enterica Typhimurium in Legon-18 pepper (Capsicum annuum) powder
Bernard Tawiah Odai*,**, Kwaku Tano-Debrah*, Kennedy Kwasi Addo***, Firibu K Saalia*, Lorenzo Moses Akyeh***
*Department of Nutrition and Food Science, University of Ghana, Legon, Accra, **Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, Kwabenya, Accra ***Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra
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摘要:

【目的】香料是一种会受到各种致病菌污染的低水分食品,其中,晒干的Legon-18辣椒粉也不例外。由于Legon-18辣椒粉会受多种致病菌的污染,所以本文研究了γ射线辐照对Legon-18辣椒粉的去污染效果和其他指标的影响。

【方法】从加纳东部地区的一位农民那里购进样品原料,并对其进行无菌检查。对样本接种已知的cfu/ml大肠杆菌、单核细胞增生李斯特菌和沙门氏菌,且分别在1、2、4和5 kGy下进行辐照,0 kGy作为对照(未照射)。所有样品在4 ℃下保存60天。在适当的培养基中对各种致病菌进行计数。以5 kGy和0 kGy为对照,对未灭菌样品进行辐照处理,确定了参数。用高效液相色谱法对辣椒素和类胡萝卜素进行了定量分析。样品在4 ℃下保存8周。

【结果】致病菌灭活呈剂量依赖性(P<0.05)。贮藏时间对病原菌的灭活也有影响(P<0.05)。在5 kGy照射后第0天,致病菌完全灭活。与未照射样品相比,照射5kGy样品的辣椒素、二氢辣椒素和辣椒素总含量分别增加了23.64%、14.7%和20.95%。γ射线照射剂量为5kGy时,辣椒红素、β-胡萝卜素和β-隐黄素分别损失8.11%、8.67%和26.54%。质量参数随贮藏时间的延长而降低(P<0.05)。

【结论】5 kGy剂量的γ射线照射可灭活致病菌。在研究过程中使用较低的辐照剂量也可以灭活致病菌,但需一定时间。各品质指标及类胡萝卜素含量受γ射线照射和贮藏时间的影响(P<0.05)。

关键词: 辣椒素类γ射线致病菌类胡萝卜素    
Abstract: Objectives
Spices are low moisture foods which have been known to be contaminated with various pathogens and sun-dried Legon-18 pepper powder is not left out. Due to its contamination with various pathogens, a study was conducted to determine the effects of gamma irradiation on the decontamination of Legon-18 pepper powder and on some quality parameters.

Methods
Samples were obtained from a local farmer from the Eastern Region of Ghana. Sterility tests were carried out. The samples were inoculated with known cfu/ml of Escherichia coli, Listeria monocytogenes and Salmonella enterica Typhimurium. Samples were irradiated at 1, 2, 4, and 5 kilogray (kGy). Zero kilogray served as control (unirradiated). All samples were stored at 4oC for 60 days. Enumeration of the various pathogens was done in appropriate media. Some quality parameters were determined after irradiating unsterile samples at 5 kGy and 0 kGy served as control. Capsaicinoids and carotenoids were quantified using a high performance liquid chromatography. The samples were stored at 4oC for 8 weeks.

Results
A dose-dependent effect on the inactivation of the pathogens was observed (P < 0.05). Storage time affected the inactivation of the pathogens as well (P < 0.05). Complete inactivation of the pathogens was observed at 5 kGy at day 0. Capsaicin, dihydrocapsaicin and total capsaicinoid content of the samples irradiated at 5 kGy increased at 23.64%, 14.7 % and 20.95% respectively as compared with the contents of the unirradiated samples. A gamma irradiation dose of 5 kGy caused losses of 8.11%, 8.67% and 26.54% in capsanthin, beta carotene and beta cryptoxanthin respectively. Quality parameters measured reduced with storage (P < 0.05).

Conclusions
Gamma irradiation inactivated pathogens at 5 kGy. Lower doses used during the study could inactivate the pathogens but with time. All quality parameters and carotenoids quantified were affected by gamma irradiation and storage period (P < 0.05).
Key words: capsaicinoids    gamma irradiation    pathogens    carotenoids
收稿日期: 2019-12-07 出版日期: 2020-03-02
通讯作者: Bernard Tawiah Odai     E-mail: bernardtodai@gmail.com
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Bernard Tawiah Odai
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Lorenzo Moses Akyeh

引用本文:

Bernard Tawiah Odai, Kwaku Tano-Debrah, Kennedy Kwasi Addo, Firibu K Saalia, Lorenzo Moses Akyeh. Effect of gamma radiation and storage at 4°C on the inactivation of Listeria monocytogenes, Escherichia coli and Salmonella enterica Typhimurium in Legon-18 pepper (Capsicum annuum) powder. Food Qual Safet, 2019, 3(4): 265-272.

链接本文:

http://www.zjujournals.com/fqs/CN/10.1093/fqsafe/fyz026        http://www.zjujournals.com/fqs/CN/Y2019/V3/I4/265

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