doi:10.3785/j.issn.1008-9209.2009.04.004

2009, Vol. 35

Issue (4): 377-382 DOI: 10.3785/j.issn.1008-9209.2009.04.004

Biological sciences & biotechnology

HAO Wen-Bo, YANG Xiao-Yu, LI Xin-Hua, GU Wen-Feng, HU Lan

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Published: 27 July 2009

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	Articles by authors
	HAO Wen-Bo
	YANG Xiao-Yu
	LI Xin-Hua
	GU Wen-Feng
	HU Lan

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HAO Wen-Bo, YANG Xiao-Yu, LI Xin-Hua, GU Wen-Feng, HU Lan. . , 2009, 35(4): 377-382.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2009.04.004 OR https://www.zjujournals.com/agr/Y2009/V35/I4/377

一种新的番鸭MSTN基因转录本的克隆及真核表达

利用RT-PCR扩增 MSTN cDNA全序列,然后进行克隆、测序;将重组质粒 pEGFP-N1-MSTN-2通过脂质体瞬时转染番鸭成纤维细胞,用RT-PCR和SDS-PAGE检测其蛋白表达.结果表明,番鸭体内存在2种MSTN基因转录本,MSTN-1(EU336991)和MSTN-2(EU336992),大小分别为1128 bp和754 bp,同源性为94%;且MSTN-2是一个全新的MSTN转录本,含有1个开放阅读框,可编码124个氨基酸.将pEGFP-N1-MSTN-2转染番鸭成纤维细胞48 h后,在荧光显微镜下可观察到绿色荧光蛋白表达,蛋白大小约为14 kDa.这对于进一步研究MSTN基因功能及作用机理具有重要意义.

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