Please wait a minute...
浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2006, Vol. 32 Issue (5): 505-510    DOI:
    
Download: HTML ( )    PDF(484KB)
Export: BibTeX | EndNote (RIS)       Supporting Info
Published: 27 September 2006
CLC:  null  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Cite this article:

. null. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 505-510.

URL:

https://www.zjujournals.com/agr/     OR     https://www.zjujournals.com/agr/Y2006/V32/I5/505


Lactobacillus reuteri PYR8亚油酸异构酶基因在毕赤酵母中的表达 　

以Lactobacillus reuteri PYR8菌株基因组DNA为模板,利用PCR方法扩增出亚油酸异构酶(linoleate isomerase,LI)基因,将其克隆至酵母表达载体pPIC9K中,电转化至毕赤酵母GS115中.重组菌株GS115/pPIC9K-LI经1%甲醇诱导后,SDS-PAGE电泳表明该基因在酵母中得到表达,即分子量约为67 kD的重组LI蛋白.经SP-Sepharose Fast Flow阳离子交换层析、Phenyl Sepharose Fast Flow疏水层析纯化获得了纯度为95%的重组LI蛋白样品.气相色谱分析表明,纯化的重组LI具有亚油酸异构酶的活性,酶的比活力为6.8 U·mg-1.
null
[1] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(6): 658-664.
[2] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 473-478.
[3] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 489-494.
[4] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 500-504.
[5] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 511-514.
[6] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 511-519.
[7] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 551-556.
[8] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 377-382.
[9] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 444-448.
[10] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 449-454.
[11] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 455-459.
[12] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 460-466.
[13] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 467-472.
[14] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 391-395.
[15] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 396-400.