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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2006, Vol. 32 Issue (5): 473-478    DOI:
    
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Published: 27 September 2006
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. null. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 473-478.

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https://www.zjujournals.com/agr/     OR     https://www.zjujournals.com/agr/Y2006/V32/I5/473


白菜雄性不育相关基因BcMF3功能的反义RNA验证

为了研究白菜等十字花科植物花粉发育及雄性不育发生的机理,根据编码果胶甲酯酶的白菜雄性不育相关基因BcMF3的cDNA序列设计特异引物,从普通白菜(Brassica campestris ssp.chinensis var.communis,syn.B.rapa ssp.chinensis var.communis)花蕾cDNA中扩增出458 bp的片断,构建BcMF3的反义RNA植物表达栽体,然后转化菜心(B. campestris ssp.chinensis var.parachinensis).研究发现,31.3%菜心转基因植株花粉表现出畸形,而且只有部分花粉具有活力,花粉离体萌发率降低为31.6%,其花药果胶甲酯酶活性降低了12.8%.上述结果表明BcMF3基因与普通白菜和菜心等白菜植物的花粉发育密切相关.
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