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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2006, Vol. 32 Issue (1): 96-100    DOI:
    
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Published: 27 January 2006
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. null. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(1): 96-100.

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https://www.zjujournals.com/agr/     OR     https://www.zjujournals.com/agr/Y2006/V32/I1/96


重组细胞色素P450 BM-3酶的表达条件及初步纯化研究

对重组细胞色素P450 BM-3基因工程菌的培养条件进行了单因素优化,确定了较佳的P450BM-3培养和表达条件:接种量1%,装液量100 mL/500 mL,发酵培养基加入0.1 mg·L-1FeCl3,OD578达到1.0左右时升温至42℃诱导,诱导时间为5 h.考察了DEAE-Sepharose FF与Resource Q 2种阴离子介质对分离纯化P450 BM-3的影响.结果表明,Resource Q纯化效果优于DEAE-Sepharose FF,通过AKTA explorer100对Resource Q分离条件进行了优化,0.3 mol·L-1、0.5 mol·L-1的两步NaCl阶跃洗脱,P450 BM-3纯度较高,活性收率为30.1%,纯化倍数为2.12.
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[2] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 511-514.
[3] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 511-519.
[4] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 551-556.
[5] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 473-478.
[6] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 489-494.
[7] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 500-504.
[8] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(5): 505-510.
[9] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 444-448.
[10] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 449-454.
[11] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 455-459.
[12] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 460-466.
[13] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 467-472.
[14] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 391-395.
[15] . null[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2006, 32(4): 396-400.