Intraguild predation between <i>Pardosa pseudoannulata</i> and <i>Paederus fuscipes</i> in ratoon rice fields and its influencing factors

doi:10.3785/j.issn.1008-9209.2022.07.041

Journal of Zhejiang University (Agriculture and Life Sciences)

2022, Vol. 48

Issue (6): 807-822 DOI: 10.3785/j.issn.1008-9209.2022.07.041

Research articles

Intraguild predation between Pardosa pseudoannulata and Paederus fuscipes in ratoon rice fields and its influencing factors

Jiaqi SUN¹(

),Chen WANG¹(

),Guanghua WANG²,Yingdang REN²,Zengrong ZHU³,Yaoyu BAI¹(

)

^1.College of Plant Protection, Southwest University, Chongqing 400715, China
^2.Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
^3.Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

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Abstract

The intraguild predation (IGP) level among the main generalist natural enemies is related to their population occurrence and the effectiveness of “green control” strategies for insect pests in agroecosystem. In this study, the detection systems of quantitative real-time polymerase chain reaction (qPCR) probe were first established and then optimized for analyzing the DNA of Pardosa pseudoannulata and Paederus fuscipes in ratoon rice fields. Then the detection systems were used to analyze the IGP levels between the two predators in ratoon rice fields. On this basis, the IGP and their biological and abiotic factors affecting the IGP level were studied under indoor conditions. The results showed that the primers and probes designed for the two predators had strong specificity. The established and optimized qPCR detection systems had good amplification repeatability and high sensitivity for the target genes of the two predators. After the system was used to detect 1 527 individuals of the two predators from ratoon rice fields and the indoor IGP tests, it was found that there was a ubiquitous and strong bidirectional IGP between the two predators in the rice field. The role transformation of intraguild predator or prey in the IGP and the influence of ambient temperature on the IGP level were closely related to their relative body sizes (developmental stages). The IGP level can be affected through extraguild preys and abiotic factors and their combination to various extents. These results enrich the IGP theory among arthropod predators in the rice ecosystem, and lay good foundations for further IGP study of natural enemies.

Key words： predatory natural enemy DNA detection rate of intraguild prey quantitative real-time polymerase chain reaction Homidia tiantaiensis ambient temperature intraguild predation level

Received: 04 July 2022 Published: 25 December 2022

CLC:

S 476.2

Corresponding Authors: Yaoyu BAI E-mail: sjq031015@163.com;w05240813@163.com;yybai711@163.com

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Cite this article:

Jiaqi SUN,Chen WANG,Guanghua WANG,Yingdang REN,Zengrong ZHU,Yaoyu BAI. Intraguild predation between Pardosa pseudoannulata and Paederus fuscipes in ratoon rice fields and its influencing factors. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(6): 807-822.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.07.041 OR https://www.zjujournals.com/agr/Y2022/V48/I6/807

再生稻田拟环纹豹蛛与青翅蚁形隐翅甲间的集团内捕食及其影响因素

重要广食性天敌间的集团内捕食（intraguild predation, IGP）水平关乎着农田系统其种群的发生和“绿色防控”策略的成效。本研究以再生稻田主要捕食者拟环纹豹蛛（Pardosa pseudoannulata）和青翅蚁形隐翅甲（Paederus fuscipes）为试验对象，首次建立和优化了实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction, qPCR）探针法检测系统，利用该系统分析了再生稻田中这2种天敌间的IGP水平，并在室内条件下剖析了该IGP及其生物和非生物影响因素。结果表明：针对这2种天敌靶基因设计的引物和探针特异性强，建立和优化的qPCR探针法系统对靶基因的扩增重复性好且灵敏度高。通过该系统对再生稻田2种天敌1 527头个体的检测及室内IGP试验发现，这2种天敌间存在着普遍且较强的双向IGP作用，其中，捕食者和猎物角色的转化以及环境温度对该IGP水平的影响均与它们之间相对体型大小（发育阶段）密切相关；集团外猎物和非生物因素及其结合能不同程度地影响IGP水平。这些研究结果丰富了稻田系统捕食者间的IGP理论体系，为深入研究稻田捕食性天敌间IGP奠定了坚实的基础。

关键词： 捕食性天敌, 集团内猎物DNA检出率, 实时荧光定量聚合酶链反应, 天台刺齿跳虫, 环境温度, 集团内捕食水平

Fig. 1 Diagram of rice straw layout

物种 Species	基因 Gene	引物与探针序列（5 $′$ →3 $′$ ） Primer and probe sequences (5 $′$ →3 $′$ )	扩增序列长度 Amplified sequence length/bp	GenBank登录号 GenBank accession No.
拟环纹豹蛛 Pp	COⅠ	F: CTTTTCTATTGATATTCCAGTCACC	153	MZ149257、 MZ149265
		R: CCACCGTTAGCGTCTACRG
		Probe: Texas Red-CCCCAGCCAAATGAAGAGAAAA-BHQ2
青翅蚁形隐翅甲 Pf	ITS	F: GGCGAAAGTAATCTGTTC	139	MZ172410
		R: CGTTCAGGGTAATCTTTTAG
		Probe: FAM-AGGTCCTCCGCTTACATCGC-MGB

Table 1 Information for primers and probes of qPCR detection system

Fig. 2 Amplification curves of qPCR detection systems of Pf and Pp

Table 2 Amplification repeatability analysis of qPCR detection systems established and optimized for the two predators

Table 3 Analysis of the body length of predators used for determination of intraguild prey DNA in the IGP

Fig. 3 DNA detection rates of intraguild prey in the IGP between Pp and PfA. Adult Pfpreying on Pp;B.Middle-instar juvenile Pp preying on Pf;C. Subadult/adult Pp preying on Pf. The same as Fig. 4.Double asterisks (**) indicate highly significant differences of DNA detection rates at the 0.01 probability level (chi-square test).

Table 4 DNA copies of intraguild prey in the guts of Pp and Pf

Fig. 4 Correlations between intraguild prey DNA detection rates in the IGP of Pp and Pf and the changes of ambient temperatures

Fig. 5 Effects of the ambient temperatures on intraguild prey DNA detection rates in the IGP between Pp and PfIn the fitting equation, D represents the DNA detection rate of intraguild prey, and T represents the ambient temperature.

Fig. 6 IGP analysis between non-starvationadult Pf and adult Pp of starvation (A) and non-starvation (B) treatments in Petri dish coexistence systems at 22 ℃Single asterisk (*) indicates significant differences between two groups at the same testing time at the 0.05 probability level (Mann-Whitney U test).

Fig. 7 IGP analysis between non-starvation low-instarjuvenile Pp andadult Pf of starvation (A₁-A₂) and non-starvation (B₁-B₂) treatments in Petri dish coexistence systems at 22 ℃Single asterisk (*) and double asterisks (**) indicate significant differences between two groups at the same testing time at the 0.05 and 0.01 probability levels, respectively (Mann-Whitney U test).

Table 5 IGP analysis between Pp and Pf in Petri dish coexistence systems at 22 ℃

Fig. 8 Effects of different factors on the survival rates of intraguild prey in the IGP of low-instar juvenile Ppandadult Pf


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