|
|
Advances on whole genome sequencing in Triticeae |
Liuhui KUANG(),Qi LI,Guoping ZHANG() |
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China |
|
|
Abstract The Triticeae provides the important cereal crops, such as wheat, barley, and rye, which produces approximately 9×108 t annually, accounting for about 30% of the total global cereal production. However, Triticeae genomes are relatively difficult to be de novo sequenced and assembled due to their large genome size, a high proportion of repeat sequences, and different ploidy levels. With the rapid development of third-generation long read sequencing technologies and assembly algorithms designed for complex genomes, and also the falling cost of genome sequencing in recent years, more and more Triticeae species have been successfully sequenced. In this study, we reviewed the advances on the whole genome sequencing of 17 Triticeae species (including subspecies), including Triticum, Hordeum, Secale, Elytrigia, and Aegilops, in aspects of sequencing technology, assembly strategy and quality, and the major research contents associated with genomes and gene functions. This review may provide the references for sequencing strategies and genomic studies of other more complex plant genomes.
|
Received: 16 May 2022
Published: 07 March 2023
|
|
Corresponding Authors:
Guoping ZHANG
E-mail: kuangliuhui@zju.edu.cn;Zhanggp@zju.edu.cn
|
小麦族全基因组测序研究进展
小麦族是禾本科植物中最重要的粮食作物来源之一,包括小麦、大麦、黑麦等麦类作物,全球年产量高达9亿t,约占全部谷类产量的30%。小麦族物种基因组庞大,重复序列比例高,且倍性水平多样,因此其从头测序和组装难度相对较大。近年来,随着三代长读长测序技术和针对复杂基因组的组装算法不断发展,以及测序成本显著下降,越来越多的小麦族物种的全基因组测序工作相继完成。本文综述了小麦族中小麦属、大麦属、黑麦属、偃麦草属和山羊草属共计17个物种(包括亚种)的全基因组研究进展,包括测序技术、拼装策略、组装质量、基因组和基因功能的主要分析内容等,旨在为更复杂的植物基因组测序和基因组学研究提供一定的理论与技术参考。
关键词:
小麦族,
基因组,
全基因组测序,
小麦属,
大麦属,
黑麦属,
偃麦草属,
山羊草属
|
|
[1] |
SUN Y Q, SHANG L G, ZHU Q H, et al. Twenty years of plant genome sequencing: achievements and challenges[J]. Trends in Plant Science, 2022, 27(4): 391-401. DOI: 10.1016/j.tplants.2021.10.006
doi: 10.1016/j.tplants.2021.10.006
|
|
|
[2] |
FEUILLET C, SALSE J. Comparative genomics in the Triticeae[M]//FEUILLET C, MUEHLBAUER G J. Genetics and Genomics of the Triticeae. Plant Genetics and Genomics: Crops and Models. Vol. 7. YorkNew, USA: Springer, 2009: 451-477. DOI: 10.1007/978-0-387-77489-3
doi: 10.1007/978-0-387-77489-3
|
|
|
[3] |
Food and Agriculture Organization of the United Nations. FAOSTAT[EB/OL]. [2022-01-03].
|
|
|
[4] |
AVNI R, NAVE M, BARAD O, et al. Wild emmer genome architecture and diversity elucidate wheat evolution and domestication[J]. Science, 2017, 357(6346): 93-96. DOI: 10.1126/science.aan0032
doi: 10.1126/science.aan0032
|
|
|
[5] |
LIU M, LI Y, MA Y L, et al. The draft genome of a wild barley genotype reveals its enrichment in genes related to biotic and abiotic stresses compared to cultivated barley[J]. Plant Biotechnology Journal, 2020, 18(2): 443-456. DOI: 10.1111/pbi.13210
doi: 10.1111/pbi.13210
|
|
|
[6] |
SATO K, MASCHER M, HIMMELBACH A, et al. Chromosome-scale assembly of wild barley accession “OUH602”[J]. G3: Genes Genomes Genetics, 2021, 11(10): jkab244. DOI: 10.1093/g3journal/jkab244
doi: 10.1093/g3journal/jkab244
|
|
|
[7] |
WANG H W, SUN S L, GE W Y, et al. Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat[J]. Science, 2020, 368(6493): eaba5435. DOI: 10.1126/science.aba5435
doi: 10.1126/science.aba5435
|
|
|
[8] |
傅向东,刘倩,李振声,等.小麦基因组研究现状与展望[J].中国科学院院刊,2018,33(9):909-914. DOI:10.16418/j.issn.1000-3045.2018.09.003 FU X D, LIU Q, LI Z S, et al. Research achievement and prospect development on wheat genome[J]. Bulletin of Chinese Academy of Sciences, 2018, 33(9): 909-914. (in Chinese with English abstract)
doi: 10.16418/j.issn.1000-3045.2018.09.003
|
|
|
[9] |
SHI X L, LING H Q. Current advances in genome sequencing of common wheat and its ancestral species[J]. Crop Journal, 2018, 6(1): 15-21. DOI: 10.1016/j.cj.2017.11.001
doi: 10.1016/j.cj.2017.11.001
|
|
|
[10] |
International Wheat Genome Sequencing Consortium. Shifting the limits in wheat research and breeding using a fully annotated reference genome[J]. Science, 2018, 361(6403): eaar7191. DOI: 10.1126/science.aar7191
doi: 10.1126/science.aar7191
|
|
|
[11] |
BRENCHLEY R, SPANNAGL M, PFEIFER M, et al. Analysis of the bread wheat genome using whole-genome shotgun sequencing[J]. Nature, 2012, 491(7426): 705-710. DOI: 10.1038/nature11650
doi: 10.1038/nature11650
|
|
|
[12] |
International Wheat Genome Sequencing Consortium. A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome[J]. Science, 2014, 345(6194): 1251788. DOI: 10.1126/science.1251788
doi: 10.1126/science.1251788
|
|
|
[13] |
CLAVIJO B J, VENTURINI L, SCHUDOMA C, et al. An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations[J]. Genome Research, 2017, 27(5): 885-896. DOI: 10.1101/gr.217117.116
doi: 10.1101/gr.217117.116
|
|
|
[14] |
ZIMIN A V, PUIU D, HALL R, et al. The first near-complete assembly of the hexaploid bread wheat genome, Triticum aestivum [J]. GigaScience, 2017, 6(11): gix097. DOI: 10.1093/gigascience/gix097
doi: 10.1093/gigascience/gix097
|
|
|
[15] |
ZHU T T, WANG L, RIMBERT H, et al. Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly[J]. The Plant Journal, 2021, 107(1): 303-314. DOI: 10.1111/tpj.15289
doi: 10.1111/tpj.15289
|
|
|
[16] |
CHAPMAN J A, MASCHER M, BULUC A, et al. A whole-genome shotgun approach for assembling and anchoring the hexaploid bread wheat genome[J]. Genome Biology, 2015, 16: 26. DOI: 10.1186/s13059-015-0582-8
doi: 10.1186/s13059-015-0582-8
|
|
|
[17] |
SATO K, ABE F, MASCHER M, et al. Chromosome-scale genome assembly of the transformation-amenable common wheat cultivar ‘Fielder’[J]. DNA Research, 2021, 28(3): dsab008. DOI: 10.1093/dnares/dsab008
doi: 10.1093/dnares/dsab008
|
|
|
[18] |
WALKOWIAK S, GAO L L, MONAT C, et al. Multiple wheat genomes reveal global variation in modern breeding[J]. Nature, 2020, 588(7837): 277-283. DOI: 10.1038/s41586-020-2961-x
doi: 10.1038/s41586-020-2961-x
|
|
|
[19] |
GUO W L, XIN M M, WANG Z H, et al. Origin and adaptation to high altitude of Tibetan semi-wild wheat[J]. Nature Communications, 2020, 11: 5085. DOI: 10.1038/s41467-020-18738-5
doi: 10.1038/s41467-020-18738-5
|
|
|
[20] |
MARCUSSEN T, SANDVE S R, HEIER L, et al. Ancient hybridizations among the ancestral genomes of bread wheat[J]. Science, 2014, 345(6194): 1250092. DOI: 10.1126/science.1250092
doi: 10.1126/science.1250092
|
|
|
[21] |
LI L F, ZHANG Z B, WANG Z H, et al. Genome sequences of five Sitopsis species of Aegilops and the origin of polyploid wheat B subgenome[J]. Molecular Plant, 2022, 15(3): 488-503. DOI: 10.1016/j.molp.2021.12.019
doi: 10.1016/j.molp.2021.12.019
|
|
|
[22] |
LING H Q, ZHAO S C, LIU D C, et al. Draft genome of the wheat A-genome progenitor Triticum urartu [J]. Nature, 2013, 496(7443): 87-90. DOI: 10.1038/nature11997
doi: 10.1038/nature11997
|
|
|
[23] |
LING H Q, MA B, SHI X L, et al. Genome sequence of the progenitor of wheat A subgenome Triticum urartu [J]. Nature, 2018, 557(7705): 424-428. DOI: 10.1038/s41586-018-0108-0
doi: 10.1038/s41586-018-0108-0
|
|
|
[24] |
JIA J Z, ZHAO S C, KONG X Y, et al. Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation[J]. Nature, 2013, 496(7443): 91-95. DOI: 10.1038/nature12028
doi: 10.1038/nature12028
|
|
|
[25] |
LUO M C, GU Y Q, YOU F M, et al. A 4-gigabase physical map unlocks the structure and evolution of the complex genome of Aegilops tauschii, the wheat D-genome progenitor[J]. PNAS, 2013, 110(19): 7940-7945. DOI: 10.1073/pnas.1219082110
doi: 10.1073/pnas.1219082110
|
|
|
[26] |
ZIMIN A V, PUIU D, LUO M C, et al. Hybrid assembly of the large and highly repetitive genome of Aegilops tauschii, a progenitor of bread wheat, with the MaSuRCA mega-reads algorithm[J]. Genome Research, 2017, 27(5): 787-792. DOI: 10.1101/gr.213405.116
doi: 10.1101/gr.213405.116
|
|
|
[27] |
ZHAO G Y, ZOU C, LI K, et al. The Aegilops tauschii genome reveals multiple impacts of transposons[J]. Nature Plants, 2017, 3(12): 946-955. DOI: 10.1038/s41477-017-0067-8
doi: 10.1038/s41477-017-0067-8
|
|
|
[28] |
LUO M C, GU Y Q, PUIU D, et al. Genome sequence of the progenitor of the wheat D genome Aegilops tauschii [J]. Nature, 2017, 551(7681): 498-502. DOI: 10.1038/nature24486
doi: 10.1038/nature24486
|
|
|
[29] |
MACCAFERRI M, HARRIS N S, TWARDZIOK S O, et al. Durum wheat genome highlights past domestication signatures and future improvement targets[J]. Nature Genetics, 2019, 51(5): 885-895. DOI: 10.1038/s41588-019-0381-3
doi: 10.1038/s41588-019-0381-3
|
|
|
[30] |
International Barley Genome Sequencing Consortium. A physical, genetic and functional sequence assembly of the barley genome[J]. Nature, 2012, 491(7426): 711-716. DOI: 10.1038/nature11543
doi: 10.1038/nature11543
|
|
|
[31] |
MASCHER M, GUNDLACH H, HIMMELBACH A, et al. A chromosome conformation capture ordered sequence of the barley genome[J]. Nature, 2017, 544(7651): 427-433. DOI: 10.1038/nature22043
doi: 10.1038/nature22043
|
|
|
[32] |
MONAT C, PADMARASU S, LUX T, et al. TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools[J]. Genome Biology, 2019, 20: 284. DOI: 10.1186/s13059-019-1899-5
doi: 10.1186/s13059-019-1899-5
|
|
|
[33] |
MASCHER M, WICKER T, JENKINS J, et al. Long-read sequence assembly: a technical evaluation in barley[J]. The Plant Cell, 2021, 33(6): 1888-1906. DOI: 10.1093/plcell/koab077
doi: 10.1093/plcell/koab077
|
|
|
[34] |
SATO K, TANAKA T, SHIGENOBU S, et al. Improvement of barley genome annotations by deciphering the Haruna Nijo genome[J]. DNA Research, 2016, 23(1): 21-28. DOI: 10.1093/dnares/dsv033
doi: 10.1093/dnares/dsv033
|
|
|
[35] |
SAKKOUR A, MASCHER M, HIMMELBACH A, et al. Chromosome-scale assembly of barley cv. ‘Haruna Nijo’ as a resource for barley genetics[J]. DNA research, 2022, 29(1): dsac001. DOI: 10.1093/dnares/dsac001
doi: 10.1093/dnares/dsac001
|
|
|
[36] |
XU W, TUCKER J R, BEKELE W A, et al. Genome assembly of the Canadian two-row malting barley cultivar AAC Synergy[J]. G3: Genes Genomes Genetics, 2021, 11(4): jkab031. DOI: 10.1093/g3journal/jkab031
doi: 10.1093/g3journal/jkab031
|
|
|
[37] |
SCHREIBER M, MASCHER M, WRIGHT J, et al. A genome assembly of the barley ‘transformation reference’ cultivar Golden Promise[J]. G3: Genes Genomes Genetics, 2020, 10(6): 1823-1827. DOI: 10.1534/g3.119.401010
doi: 10.1534/g3.119.401010
|
|
|
[38] |
JAYAKODI M, PADMARASU S, HABERER G, et al. The barley pan-genome reveals the hidden legacy of mutation breeding[J]. Nature, 2020, 588(7837): 284-289. DOI: 10.1038/s41586-020-2947-8
doi: 10.1038/s41586-020-2947-8
|
|
|
[39] |
ZENG X Q, LONG H, WANG Z, et al. The draft genome of Tibetan hulless barley reveals adaptive patterns to the high stressful Tibetan Plateau[J]. PNAS, 2015, 112(4): 1095-1100. DOI: 10.1073/pnas.1423628112
doi: 10.1073/pnas.1423628112
|
|
|
[40] |
DAI F, WANG X L, ZHANG X Q, et al. Assembly and analysis of a qingke reference genome demonstrate its close genetic relation to modern cultivated barley[J]. Plant Biotech-nology Journal, 2018, 16(3): 760-770. DOI: 10.1111/pbi.12826
doi: 10.1111/pbi.12826
|
|
|
[41] |
ZENG X Q, XU T, LING Z H, et al. An improved high-quality genome assembly and annotation of Tibetan hulless barley[J]. Scientific Data, 2020, 7: 139. DOI: 10.1038/s41597-020-0480-0
doi: 10.1038/s41597-020-0480-0
|
|
|
[42] |
GARTHWAITE A J, STEUDLE E, COLMER T D. Water uptake by roots of Hordeum marinum: formation of a barrier to radial O2 loss does not affect root hydraulic conductivity[J]. Journal of Experimental Botany, 2006, 57(3): 655-664. DOI: 10.1093/jxb/erj055
doi: 10.1093/jxb/erj055
|
|
|
[43] |
GARTHWAITE A J, VON BOTHMER R, COLMER T D. Salt tolerance in wild Hordeum species is associated with restricted entry of Na+ and Cl- into the shoots[J]. Journal of Experimental Botany, 2005, 56(419): 2365-2378. DOI: 10.1093/jxb/eri229
doi: 10.1093/jxb/eri229
|
|
|
[44] |
ALAMRI S A, BARRETT-LENNARD E G, TEAKLE N L, et al. Improvement of salt and waterlogging tolerance in wheat: comparative physiology of Hordeum marinum-Triticum aestivum amphiploids with their H. marinum and wheat parents[J]. Functional Plant Biology, 2013, 40(11): 1168-1178. DOI: 10.1071/fp12385
doi: 10.1071/fp12385
|
|
|
[45] |
ISLAM S, MALIK A I, ISLAM A K M R, et al. Salt tolerance in a Hordeum marinum-Triticum aestivum amphiploid, and its parents[J]. Journal of Experimental Botany, 2007, 58(5): 1219-1229. DOI: 10.1093/jxb/erl293
doi: 10.1093/jxb/erl293
|
|
|
[46] |
KUANG L H, SHEN Q F, CHEN L Y, et al. The genome and gene editing system of sea barleygrass provide a novel platform for cereal domestication and stress tolerance studies[J]. Plant Communications, 2022, 3(5): 100333. DOI: 10.1016/j.xplc.2022.100333
doi: 10.1016/j.xplc.2022.100333
|
|
|
[47] |
LI G W, WANG L J, YANG J P, et al. A high-quality genome assembly highlights rye genomic characteristics and agrono-mically important genes[J]. Nature Genetics, 2021, 53(4): 574-584. DOI: 10.1038/s41588-021-00808-z
doi: 10.1038/s41588-021-00808-z
|
|
|
[48] |
BAUER E, SCHMUTZER T, BARILAR I, et al. Towards a whole-genome sequence for rye (Secale cereale L.)[J]. The Plant Journal, 2017, 89(5): 853-869. DOI: 10.1111/tpj.13436
doi: 10.1111/tpj.13436
|
|
|
[49] |
AVNI R, LUX T, MINZ-DUB A, et al. Genome sequences of three Aegilops species of the section Sitopsis reveal phylogenetic relationships and provide resources for wheat improvement[J]. The Plant Journal, 2022, 110(1): 179-192. DOI: 10.1111/tpj.15664
doi: 10.1111/tpj.15664
|
|
|
[50] |
YU G T, MATNY O, CHAMPOURET N, et al. Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62 [J]. Nature Communications, 2022, 13: 1607. DOI: 10.1038/s41467-022-29132-8
doi: 10.1038/s41467-022-29132-8
|
|
|
[51] |
MUNNS R, JAMES R A, XU B, et al. Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene[J]. Nature Biotechnology, 2012, 30(4): 360-364. DOI: 10.1038/nbt.2120
doi: 10.1038/nbt.2120
|
|
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|