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Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (2): 261-268    DOI: 10.3785/j.issn.1008-9209.2021.12.061
Animal sciences & veterinary medicines     
Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Holstein cows in southern China
Chenhui LIU1(),Hailong WU2(),Lei CHENG1(),Jie YU1,Yu XIA1,Min XIANG1,Xiuzhong HU1,Dingfa WANG1,Bifei TAO1
1.Wuhan Academy of Agricultural Sciences, Wuhan 430065, Hubei, China
2.Wuhan Bright Dairy Ecological Demonstration Dairy Farm Co. , Ltd. , Wuhan 430045, Hubei, China
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Abstract  

This study aimed to investigate the effects of FYN binding protein (FYB) gene mutation on lactation performance of Holstein cows in southern China. The lactation traits of 785 Holstein cows from 2014 to 2020 were collected, and the single nucleotide polymorphisms (SNPs) in FYB gene were detected by SNaPshot and the effects of the mutation on structural and physicochemical properties of FYB protein were analyzed. The associations between the mutations and lactation traits and serum biochemical indexes were analyzed by the least square method. The results showed that the allele frequencies of C and T at rs109262355 in FYB gene were 0.36 and 0.64, respectively, which were consistent with Hardy-Weinberg equilibrium (P>0.05). An extra hydrogen bond with a length of 3.34 ? in the related region of FYB protein caused by the mutation from C to T at rs109262355 was formed, which reduced the hydrophobicity and flexibility of the protein in this region. 305 d milk yield, peak milk yield, and milk fat rate between CC and TT genotype individuals in rs109262355 was significantly different (P<0.05), with the dominant allele T of milk yield and the dominant allele C of milk fat rate. The serum low-density lipoprotein (LDL) was significantly different between CC and TT genotype individuals (P<0.05); alkaline phosphatase (ALP) and total cholesterol (TC) in serum were highly significantly different between CC and TT genotype individuals (P<0.01). The rs109262355 mutation in FYB gene can affect the milk yield and milk fat percentage of dairy cows, which could be used as a candidate genetic marker in lactation traits of Holstein cows in southern China of marker-assisted selection breeding.



Key wordsHolstein cows      FYN binding protein gene      lactation performance      allele     
Received: 06 December 2021      Published: 25 April 2023
CLC:  S823.91  
Corresponding Authors: Lei CHENG     E-mail: lchhhcl890621@sina.com;wuhailong1004@126.com;chenglei_011@126.com
Cite this article:

Chenhui LIU,Hailong WU,Lei CHENG,Jie YU,Yu XIA,Min XIANG,Xiuzhong HU,Dingfa WANG,Bifei TAO. Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Holstein cows in southern China. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 261-268.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.12.061     OR     https://www.zjujournals.com/agr/Y2023/V49/I2/261


FYN结合蛋白基因rs109262355突变对中国南方荷斯坦牛泌乳性能的影响

为探究FYN结合蛋白(FYN binding protein,FYB)基因突变对中国南方荷斯坦牛泌乳性能的影响,以785头中国南方地区荷斯坦牛为研究对象,收集其2014—2020年的泌乳性状数据,通过SNaPshot技术对FYB基因突变位点进行单核苷酸多态性检测,分析突变对FYB蛋白结构和理化性质的影响,并利用最小二乘法将突变与奶牛泌乳性状、血清生化指标进行关联分析。结果显示:在试验群体中,FYB基因rs109262355位点等位基因C和T的频率分别为0.36和0.64,符合哈代-温伯格(Hardy-Weinberg)平衡(P>0.05);该位点由C→T的突变会导致FYB蛋白对应区域形成1个额外的长度为3.34 ?的氢键,同时使该区域的蛋白疏水性和柔性降低;该位点CC和TT基因型个体的305 d产奶量、高峰产奶量及乳脂率均存在显著差异(P<0.05),等位基因T为产奶量优势等位基因,等位基因C为乳脂率优势等位基因;该位点CC和TT基因型个体血清中低密度脂蛋白(low-density lipoprotein, LDL)含量存在显著差异(P<0.05),碱性磷酸酶(alkaline phosphatase, ALP)活性和总胆固醇(total cholesterol, TC)含量存在极显著差异(P<0.01)。FYB基因rs109262355突变能够影响奶牛产奶量及乳脂率,可作为候选遗传标记应用于中国南方荷斯坦牛泌乳性状的标记辅助选择育种。


关键词: 荷斯坦牛,  FYN结合蛋白基因,  泌乳性能,  等位基因 
类型 TypeFYB基因 FYB gene类型 TypeFYB蛋白 FYB protein
染色体定位 On chromosome20:35233378
编码区定位 On coding region653蛋白质定位 On protein第218位氨基酸
等位基因 AlleleC/T氨基酸 Amino acid丙氨酸/缬氨酸
密码子 CodeGCG/GTG
突变类型 Mutation type错义突变
Table 1 Details of rs109262355 mutation in FYB gene

基因型

Genotype

个体数

Individual number

基因型频率

Genotype frequency

等位基因

Allele

等位基因频率

Allele frequency

χ2

p

p-value

CC980.13C0.360.1680.919
CT3700.47T0.64
TT3170.40
Table 2 Distribution of rs109262355 mutation in trialed group
Fig. 1 Effects of rs109262355 mutation on the structures and physicochemical properties of FYB proteinA. Conserved domains and three-dimensional structure models of FYB protein (the amino acid corresponding to rs109262355 is marked with orange, and the PHA03247 superfamily, SH3 superfamily, and hSH3 conserved domains are marked with blue, green, and red, respectively) B-C. Three-dimensional structure models of 189-289 amino acids when the nucleotides of rs109262355 were alleles of C and T, respectively. D-E. Effects of rs109262355 on hydrophobicity and flexibility of FYB protein when the nucleotide C (FYB-O) mutates to T (FYB-M).
泌乳性状 Lactation trait基因型 Genotype表型值 Phenotypic valueFF valuepp-value

泌乳天数

Lactation day/d

CC326.79±6.381.070.34
CT323.82±4.16
TT318.84±4.43

305 d产奶量

305 d milk yield/kg

CC9 731.60±143.80b3.790.02*
CT10 136.75±93.66a
TT10 074.23±99.80a

总奶量

Total milk yield/kg

CC10 166.48±257.320.870.42
CT10 516.82±164.87
TT10 401.18±177.97

高峰产奶量

Peak milk yield/kg

CC43.69±0.57b3.270.04*
CT44.84±0.37ab
TT45.27±0.40a

高峰日

Peak day/d

CC90.29±4.400.510.60
CT94.36±2.82
TT91.97±3.05

乳脂率

Milk fat percentage/%

CC4.53±0.04A4.400.01*
CT4.45±0.03AB
TT4.40±0.03B

乳蛋白率

Milk protein percentage/%

CC3.32±0.021.750.17
CT3.29±0.01
TT3.28±0.01

体细胞数

Somatic cell count/(104/mL)

CC11.49±1.110.650.52
CT12.11±0.71
TT12.75±0.77
Table 3 Effects of rs109262355 mutation on lactation traits in trialed group
血清生化指标 Serum biochemical index基因型 Genotype表型值 Phenotypic valueFF valuepp-value

碱性磷酸酶活性

ALP activity/(U/L)

CC55.30±2.56B8.830.000 2**
CT63.92±1.36A
TT67.69±1.48A

ρ(总胆固醇)

TC content/(mg/dL)

CC223.91±8.90B4.730.009 1**
CT224.40±4.65B
TT244.42±5.08A

ρ(低密度脂蛋白)

LDL content/(mg/dL)

CC158.29±8.65b4.200.015 3*
CT159.89±4.53b
TT178.03±4.97a
Table 4 Effects of rs109262355 mutation on serum biochemical indexes in trialed group
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