Animal sciences & veterinary medicines |
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Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Holstein cows in southern China |
Chenhui LIU1(),Hailong WU2(),Lei CHENG1(),Jie YU1,Yu XIA1,Min XIANG1,Xiuzhong HU1,Dingfa WANG1,Bifei TAO1 |
1.Wuhan Academy of Agricultural Sciences, Wuhan 430065, Hubei, China 2.Wuhan Bright Dairy Ecological Demonstration Dairy Farm Co. , Ltd. , Wuhan 430045, Hubei, China |
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Abstract This study aimed to investigate the effects of FYN binding protein (FYB) gene mutation on lactation performance of Holstein cows in southern China. The lactation traits of 785 Holstein cows from 2014 to 2020 were collected, and the single nucleotide polymorphisms (SNPs) in FYB gene were detected by SNaPshot and the effects of the mutation on structural and physicochemical properties of FYB protein were analyzed. The associations between the mutations and lactation traits and serum biochemical indexes were analyzed by the least square method. The results showed that the allele frequencies of C and T at rs109262355 in FYB gene were 0.36 and 0.64, respectively, which were consistent with Hardy-Weinberg equilibrium (P>0.05). An extra hydrogen bond with a length of 3.34 ? in the related region of FYB protein caused by the mutation from C to T at rs109262355 was formed, which reduced the hydrophobicity and flexibility of the protein in this region. 305 d milk yield, peak milk yield, and milk fat rate between CC and TT genotype individuals in rs109262355 was significantly different (P<0.05), with the dominant allele T of milk yield and the dominant allele C of milk fat rate. The serum low-density lipoprotein (LDL) was significantly different between CC and TT genotype individuals (P<0.05); alkaline phosphatase (ALP) and total cholesterol (TC) in serum were highly significantly different between CC and TT genotype individuals (P<0.01). The rs109262355 mutation in FYB gene can affect the milk yield and milk fat percentage of dairy cows, which could be used as a candidate genetic marker in lactation traits of Holstein cows in southern China of marker-assisted selection breeding.
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Received: 06 December 2021
Published: 25 April 2023
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Corresponding Authors:
Lei CHENG
E-mail: lchhhcl890621@sina.com;wuhailong1004@126.com;chenglei_011@126.com
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Cite this article:
Chenhui LIU,Hailong WU,Lei CHENG,Jie YU,Yu XIA,Min XIANG,Xiuzhong HU,Dingfa WANG,Bifei TAO. Effect of rs109262355 mutation in FYN binding protein gene on lactation performance of Holstein cows in southern China. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(2): 261-268.
URL:
https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.12.061 OR https://www.zjujournals.com/agr/Y2023/V49/I2/261
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FYN结合蛋白基因rs109262355突变对中国南方荷斯坦牛泌乳性能的影响
为探究FYN结合蛋白(FYN binding protein,FYB)基因突变对中国南方荷斯坦牛泌乳性能的影响,以785头中国南方地区荷斯坦牛为研究对象,收集其2014—2020年的泌乳性状数据,通过SNaPshot技术对FYB基因突变位点进行单核苷酸多态性检测,分析突变对FYB蛋白结构和理化性质的影响,并利用最小二乘法将突变与奶牛泌乳性状、血清生化指标进行关联分析。结果显示:在试验群体中,FYB基因rs109262355位点等位基因C和T的频率分别为0.36和0.64,符合哈代-温伯格(Hardy-Weinberg)平衡(P>0.05);该位点由C→T的突变会导致FYB蛋白对应区域形成1个额外的长度为3.34 ?的氢键,同时使该区域的蛋白疏水性和柔性降低;该位点CC和TT基因型个体的305 d产奶量、高峰产奶量及乳脂率均存在显著差异(P<0.05),等位基因T为产奶量优势等位基因,等位基因C为乳脂率优势等位基因;该位点CC和TT基因型个体血清中低密度脂蛋白(low-density lipoprotein, LDL)含量存在显著差异(P<0.05),碱性磷酸酶(alkaline phosphatase, ALP)活性和总胆固醇(total cholesterol, TC)含量存在极显著差异(P<0.01)。FYB基因rs109262355突变能够影响奶牛产奶量及乳脂率,可作为候选遗传标记应用于中国南方荷斯坦牛泌乳性状的标记辅助选择育种。
关键词:
荷斯坦牛,
FYN结合蛋白基因,
泌乳性能,
等位基因
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|
[1] |
马彦男,贺鹏迦,朱静,等. GHR基因F279Y位点突变对中国荷斯坦牛泌乳性状的影响[J].中国应用生理学杂志,2013,29(5):400-404. DOI:10.13459/j.cnki.cjap.2013.05.001 MA Y N, HE P J, ZHU J, et al. The effect of polymorphism F279Y of GHR gene on milk production trait in Chinese Holstein cattle[J]. Chinese Journal of Applied Physiology, 2013, 29(5): 400-404. (in Chinese with English abstract)
doi: 10.13459/j.cnki.cjap.2013.05.001
|
|
|
[2] |
BOROWICZ P, CHAN H, HAUGE A, et al. Adaptor proteins: flexible and dynamic modulators of immune cell signalling[J]. Scandinavian Journal of Immunology, 2020, 92(5): e12951. DOI: 10.1111/sji.12951
doi: 10.1111/sji.12951
|
|
|
[3] |
VINEETH M R, SURYA T, SIVALINGAM J, et al. Genome-wide discovery of SNPs in candidate genes related to production and fertility traits in Sahiwal cattle[J]. Tropical Animal Health and Production, 2020, 52: 1707-1715. DOI: 10.1007/s11250-019-02180-x
doi: 10.1007/s11250-019-02180-x
|
|
|
[4] |
JIANG L, LIU J F, SUN D X, et al. Genome wide association studies for milk production traits in Chinese Holstein population[J]. PLoS ONE, 2010, 5(10): e13661. DOI: 10.1371/journal.pone.0013661
doi: 10.1371/journal.pone.0013661
|
|
|
[5] |
MARETE A G, GULDBRANDTSEN B, LUND M S, et al. A meta-analysis including pre-selected sequence variants associated with seven traits in three French dairy cattle populations[J]. Frontiers in Genetics, 2018, 9: 522. DOI: 10.3389/fgene.2018.00522
doi: 10.3389/fgene.2018.00522
|
|
|
[6] |
朱平,杜力杰,孟昆,等.三型分泌系统效应蛋白调控细胞凋亡和焦亡的研究进展[J].生物技术通报,2019,35(4):178-187. DOI:10.13560/j.cnki.biotech.bull.1985.2018-0897 ZHU P, DU L J, MENG K, et al. Research progress on the effects of T3SS effectors on apoptosis and pyroptosis of host cells[J]. Biotechnology Bulletin, 2019, 35(4): 178-187. (in Chinese with English abstract)
doi: 10.13560/j.cnki.biotech.bull.1985.2018-0897
|
|
|
[7] |
ŚWIDERSKA E, STRYCHARZ J, WRÓBLEWSKI A, et al. Role of PI3K/AKT pathway in insulin-mediated glucose uptake[M]//SZABLEWSKI L. Blood Glucose Levels. London, UK: IntechOpen, 2018.
|
|
|
[8] |
LU S N, WANG J Y, CHITSAZ F, et al. CDD/SPARCLE: the conserved domain database in 2020[J]. Nucleic Acids Reseach, 2020, 48(D1): D265-D268. DOI: 10.1093/nar/gkz991
doi: 10.1093/nar/gkz991
|
|
|
[9] |
KELLEY L A, STERNBERG M J E. Protein structure prediction on the Web: a case study using the Phyre server[J]. Nature Protocols, 2009, 4(3): 363-371. DOI: 10.1038/nprot.2009.2
doi: 10.1038/nprot.2009.2
|
|
|
[10] |
MERTANIASIH N M, SURYA S D D N, S SOEDARSONO et al. The espD full gene as a potential biomarker in active pulmonary tuberculosis[J]. International Journal of Mycobac-teriology, 2021, 10(4): 421-427. DOI: 10.4103/ijmy.ijmy_198_21
doi: 10.4103/ijmy.ijmy_198_21
|
|
|
[11] |
BORNOT A, ETCHEBEST C, DE BREVERN A G. Predicting protein flexibility through the prediction of local structures[J]. Proteins: Structure, Function, and Bioinformatics, 2011, 79(3): 839-852. DOI: 10.1002/prot.22922
doi: 10.1002/prot.22922
|
|
|
[12] |
WILKINS M R, GASTEIGER E, BAIROCH A, et al. Protein identification and analysis tools on the ExPASy server[M]//LINK A J. 2-D Proteome Analysis Protocols, Methods in Molecular Biology. New Jersey, USA: Humana Press, 1999: 531-552.
|
|
|
[13] |
RASOOL M, PUSHPARAJ P N, BUHMEIDA A, et al. Mutational spectrum of BRAF gene in colorectal cancer patients in Saudi Arabia[J]. Saudi Journal of Biological Sciences, 2021, 28(10): 5906-5912. DOI: 10.1016/j.sjbs.2021.06.048
doi: 10.1016/j.sjbs.2021.06.048
|
|
|
[14] |
WANG M Q, SONG H L, ZHU X R, et al. Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows[J]. Journal of Dairy Research, 2018, 85(4): 407-411. DOI: 10.1017/S0022029918000535
doi: 10.1017/S0022029918000535
|
|
|
[15] |
MCNABB D S, COURTNEY R J. Analysis of the UL36 open reading frame encoding the large tegument protein (ICP1/2) of herpes simplex virus type 1[J]. Journal of Virology, 1992, 66(12): 7581-7584.
|
|
|
[16] |
BAINES J D, ROIZMAN B. The UL10 gene of herpes simplex virus 1 encodes a novel viral glycoprotein, gM, which is present in the virion and in the plasma membrane of infected cells[J]. Journal of Virology, 1993, 67(3): 1441-1452.
|
|
|
[17] |
SCHLIEKER C, KORBEL G A, KATTENHORN L M, et al. A deubiquitinating activity is conserved in the large tegument protein of the herpesviridae [J]. Journal of Virology, 2005, 79(24): 15582-15585. DOI:10.1128/JVI.79.24.15582-15585.2005
doi: 10.1128/JVI.79.24.15582-15585.2005
|
|
|
[18] |
夏成,王洪斌,张洪友,等.能量负平衡对泌乳早期奶牛生产性能、繁殖性能和机体代谢的影响[J].中国畜牧杂志,2009,45(21):32-35. XIA C, WANG H B, ZHANG H Y, et al. Effects of negative energy balance on production performance, reproductive performance, and body metabolism of dairy cows in early lactation[J]. Chinese Journal of Animal Science, 2009, 45(21): 32-35. (in Chinese)
|
|
|
[19] |
张德利,任大卫,张德文,等.高脂饮食诱导肥胖SD大鼠体内瘦素水平及其血清胰岛素的变化[J].浙江临床医学,2006,8(4):338-339. DOI:10.3969/j.issn.1008-7664.2006.04.002 ZHANG D L, REN D W, ZHANG D W, et al. Changes of leptin level and serum insulin value in SD rats induced by high fat diet[J]. Zhejiang Clinical Medical Journal, 2006,8(4): 338-339. (in Chinese with English abstract)
doi: 10.3969/j.issn.1008-7664.2006.04.002
|
|
|
[20] |
OSWAL A, YEO G. Leptin and the control of body weight: a review of its diverse central targets, signaling mechanisms, and role in the pathogenesis of obesity[J]. Obesity, 2010, 18(2): 221-229. DOI: 10.1038/oby.2009.228
doi: 10.1038/oby.2009.228
|
|
|
[21] |
MANJUNATHAN R, DEVARAJAN N, RAGUNATHAN M. Possible mechanism of human recombinant leptin-induced VEGF A synthesis via PI3K/Akt/mTOR/S6 kinase signaling pathway while inducing angiogenesis: an analysis using chicken chorioallantoic membrane model[J]. Journal of Vascular Research, 2021, 58(6): 343-360. DOI: 10.1159/000516498
doi: 10.1159/000516498
|
|
|
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