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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (3): 389-397    DOI: 10.3785/j.issn.1008-9209.2021.11.011
Resource utilization & environmental protection     
Effect of remediation of polychlorinated biphenyls (PCBs) contaminated soil by combined treatment of nanoscale zero-valent iron and PCBs-degrading strain
Lyuyang SHAO1(),Xi CHEN2(),Chaofeng SHEN3()
1.Department of Water Resources and Environmental Engineering, Tamkang University, New Taipei City 25137, Taiwan, China
2.Shanghai Chemical Engineering Monitoring Station for Environment Protection, Shanghai 200050, China
3.College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China
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Abstract  

Polychlorinated biphenyls (PCBs) are typical pollutants in soil. Nanoscale zero-valent iron (nZVI) can effectively dechlorinate high-chlorinated PCBs into low-chlorinated PCBs which can be more easily degraded by microorganisms. In this study, soil samples contaminated by PCBs were collected from an e-waste recycling site in Taizhou, Zhejiang Province. The remediation of combined nZVI and PCBs-degrading strain Rhodococcus pyridinovorans R04 was carried out to investigate the removal effect of PCBs in soil. The results showed that the combined treatment of nZVI and degrading strain R04 had better effects than the treatment of nZVI or R04 alone in removing PCBs or various chlorinated PCB congeners in soil. After combined treatment with nZVI and degrading strain R04 for 32 d, the removal rate of PCBs in soil reached 59.4%, which was higher than 46.1% of nZVI treatment and 34.4% of degrading strain R04 treatment (P＜0.05). Therefore, the combination of nZVI and degrading bacteria in the remediation of PCBs contaminated soil has good application potential.

Key words： polychlorinated biphenyls (PCBs)      dechlorination      nanoscale zero-valent iron (nZVI)      degrading bacteria      remediation of soil pollution     
Received: 01 November 2021      Published: 25 June 2023
CLC:  X53  
Corresponding Authors: Chaofeng SHEN     E-mail: 736037122@qq.com;ysxzt@zju.edu.cn
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Lyuyang SHAO
Xi CHEN
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Cite this article:

Lyuyang SHAO,Xi CHEN,Chaofeng SHEN. Effect of remediation of polychlorinated biphenyls (PCBs) contaminated soil by combined treatment of nanoscale zero-valent iron and PCBs-degrading strain. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(3): 389-397.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.11.011     OR     https://www.zjujournals.com/agr/Y2023/V49/I3/389


纳米零价铁与降解菌联合修复多氯联苯污染土壤效果的研究

多氯联苯（polychlorinated biphenyls, PCBs）是土壤中典型的污染物，纳米零价铁（nanoscale zero-valent iron, nZVI）可以高效地将高氯代PCBs脱氯成更容易被微生物降解的低氯代PCBs。本研究从浙江省台州市某电子垃圾拆解场采集了受PCBs污染的土壤样品，采用nZVI与PCBs降解菌嗜吡啶红球菌（Rhodococcus pyridinovorans）R04开展联合修复研究，考察其对PCBs的去除效果。结果表明：nZVI与降解菌R04联合处理对土壤中PCBs总量及各氯代PCB同类物的去除效果均优于nZVI或R04单独处理；nZVI与降解菌R04联合处理32 d时，土壤中PCBs去除率达59.4%，高于单独使用nZVI的去除率（46.1%），也显著高于单独使用降解菌R04的去除率（34.4%，P＜0.05）。因此，nZVI与降解菌联合修复PCBs污染土壤具有较好的应用潜力。


关键词： 多氯联苯,  脱氯,  纳米零价铁,  降解菌,  土壤污染修复 
Fig. 1 Transmission electron microscopy image (A) and X-ray diffraction pattern (B) of nZVI

组别

Group

土样质量

Soil sample

mass/g

去离子水

Deionized

water/mL

nZVI投加量

nZVI dosage/g

T03.0300
T13.0301.0
T23.0300.5
T33.0300.1
Table 1 Soil sample mass and nZVI dosage

组别

Group

土样质量

Soil sample

mass/g

去离子水

Deionized

water/mL

R04投加量

R04 dosage/

(CFU/g)

T03.0300
R04（2×108 CFU/g）3.0302×108
R04（1×109 CFU/g）3.0301×109
R04（－）3.030—
Table 2 Soil sample mass and degrading strain R04 dosage

组别

Group

土样质量

Soil sample mass/g

去离子水

Deionized water/mL

nZVI投加量

nZVI dosage/g

R04投加量

R04 dosage/(CFU/g)

T03.03000
R04（1×109 CFU/g）3.03001×109
R04（－）3.0300—
nZVI（+）3.03010
nZVI（+）+R04（1×109 CFU/g）3.03011×109
nZVI（+）+R04（－）3.0301—
Table 3 Soil sample mass and dosages of nZVI and degrading strain R04
Fig. 2 Effects of nZVI dosages on the removal effect of PCBs in soilThe meanings of T0-T3 are the same as in Table 1, and the same as below. Different lowercase letters above bars indicate significant differences among different treatments at the same time at the 0.05 probability level, and the same as Figs. 4 and 5.
Fig. 3 Effects of nZVI dosages on the removal effect of each chlorinated PCB congener in soil on 32 dDifferent lowercase letters above bars indicate significant differences among different treatments of the same compound at the 0.05 probability level, and the same as Fig. 6.
Fig. 4 Effects of degrading strain R04 dosage on the removal effect of PCBs in soil
Fig. 5 Effects of different treatments on the removal effect of PCBs in soil
Fig. 6 Effects of different treatments on each chlorinated PCB congener residual concentration in contaminated soil
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