Protective effect and mechanism of matcha on liver lipid accumulation and inflammatory response induced by high-fat diet in mice

doi:10.3785/j.issn.1008-9209.2021.07.111

Journal of Zhejiang University (Agriculture and Life Sciences)

2022, Vol. 48

Issue (4): 525-532 DOI: 10.3785/j.issn.1008-9209.2021.07.111

Animal sciences & veterinary medicines

Protective effect and mechanism of matcha on liver lipid accumulation and inflammatory response induced by high-fat diet in mice

Jihong ZHOU¹(

),Yue'er YU¹,Lejia DING¹,Ping XU¹,Limin MAO^1,²,Yuefei WANG¹(

)

^1.Tea Research Institute, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
^2.Zhejiang Tea Group Co. , Ltd. , Hangzhou 310058, China

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Abstract

In this study, we analyzed the main chemical components of matcha made from four tea cultivars, established a high-fat diet-induced C57BL/6J obese mouse model, and selected ‘Maolü’ matcha as an experimental dietary supplement with three doses of 0.1%, 0.5% and 1.0%. The results showed that matcha could reduce body mass gain, blood glucose level rise and liver lipid accumulation induced by the high-fat diet without affecting food intake, and the effect was concentration-dependent. Furthermore, we detected the liver function, oxidative stress level and inflammatory response in mice, and the results showed that dietary supplementation of 1.0% matcha significantly inhibited the abnormal increase of activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) induced by a high-fat diet in liver, and increased the activity of antioxidant enzyme. The expression levels of inflammatory factors, Toll-like receptor 4 (TLR4) and MyD88 were also significantly reduced. In conclusion, matcha effectively improves obesity-related fatty liver lesions and inflammation, and its potential mechanism is to inhibit the activation of the TLR4/MyD88 signaling pathway.

Key words： matcha obesity non-alcoholic fatty liver disease oxidative stress inflammatory cytokines

Received: 11 July 2021 Published: 03 September 2022

CLC:

S 571.1

Corresponding Authors: Yuefei WANG E-mail: zhoujihong@zju.edu.cn;zdcy@zju.edu.cn

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	Jihong ZHOU
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	Limin MAO
	Yuefei WANG

Cite this article:

Jihong ZHOU,Yue'er YU,Lejia DING,Ping XU,Limin MAO,Yuefei WANG. Protective effect and mechanism of matcha on liver lipid accumulation and inflammatory response induced by high-fat diet in mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(4): 525-532.

URL:

https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.07.111 OR https://www.zjujournals.com/agr/Y2022/V48/I4/525

抹茶对高脂饮食诱导的小鼠肝脏脂质积累和炎症反应的保护作用及机制

本研究比较分析了4个茶树品种抹茶主要化学成分含量的差异，并选取‘茂绿’品种抹茶对高脂饮食诱导的C57BL/6J肥胖小鼠模型进行0.1%、0.5%、1.0% 3种剂量的膳食补充。结果表明，抹茶能够在不影响摄食量的情况下改善高脂饮食导致的体质量增长、血糖水平升高和肝脏脂质积累，且效果呈现出浓度依赖性。进一步对小鼠肝脏功能、氧化应激水平和炎症反应情况进行检测，发现膳食添加1.0%的抹茶能够显著抑制高脂饮食诱导的肝脏谷丙转氨酶（alanine aminotransferase, ALT）和谷草转氨酶（aspartate aminotransferase, AST）活性异常升高，提升抗氧化酶活性，降低炎症因子表达水平，并下调Toll样受体4（Toll-like receptor 4, TLR4）和下游MyD88基因的表达。综上所述，抹茶能够有效改善食源性肥胖和高脂饮食诱发的肝脏脂肪性病变与炎症反应，对TLR4/MyD88信号通路的抑制是其潜在的作用机制。

关键词： 抹茶, 肥胖, 非酒精性脂肪性肝病, 氧化应激, 炎症因子

Table 1 Primer sequences of qRT-PCR

Table 2 Contents of main biochemical components in matcha made from different tea cultivars

Table 3 Catechin monomer contents in matcha made from different tea cultivars

Table 4 Effects of matcha on body mass, food intake and fasting blood glucose level in different groups of mice

Fig. 1 Effects of matcha on liver lipid accumulation in different groups of miceIn HE staining, the nuclei of liver were stained blue, and the cytoplasm was stained red, and the lipids were not colored and appeared as white vacuoles in the micrograph. In oil red O staining, the nuclei of liver were stained blue, and the lipids were stained red.

Fig. 2 Effects of matcha on liver function and oxidative stress level in different groups of miceDifferent lowercase letters above bars indicate significant differences at the 0.05 probability level.

Fig. 3 Effects of matcha on relative expression levels of inflammatory cytokine and TLR4/MyD88 signaling pathway gene in different groups of miceDifferent lowercase letters above bars indicate significant differences among different treatments at the same inflammatory cytokine or gene at the 0.05 probability level.


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