Research progress of peroxiredoxingene in crustaceans

doi:10.3785/j.issn.1008-9209.2020.08.051

Journal of Zhejiang University (Agriculture and Life Sciences)

2021, Vol. 47

Issue (3): 284-294 DOI: 10.3785/j.issn.1008-9209.2020.08.051

Reviews

Research progress of peroxiredoxingene in crustaceans

Hui LI^1,²(

),Wei FENG^2,³,Junjie YU^2,³,Mingyin ZHANG^2,³,Chunmiao ZHOU^2,³,Yongkai TANG^1,^2,³(

)

^1.National Demonstration Center for Experimental Fisheries Science Education (Shanghai Ocean University), Shanghai 201306, China
^2.Ministry of Agriculture and Rural Affairs Key Laboratory of Freshwater Aquatic Germplasm Resources, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, Jiangsu, China
^3.Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214128, Jiangsu, China

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Abstract

Peroxiredoxin (Prx) belongs to the superfamily of antioxidant proteins, which widely exists in eukaryotes and prokaryotes, and plays an important role in innate immunity. At present, there are a few researches on the Prx genes in crustaceans, and only partial subtypes of the Prx genes are cloned, and the antioxidant effect is studied only in terms of biological function. In this study, the cloning, phylogeny, tissue expression and biological functions of crustacean Prx genes in recent years were reviewed. Furthermore, researches on Prx genes in crustaceans were prospected, which would provide references for the researches on the structures and biological functions of Prx genes in crustaceans.

Key words： crustacean peroxiredoxin gene cloning tissue expression biological function

Received: 05 August 2020 Published: 25 June 2021

CLC:

S 917.4

Corresponding Authors: Yongkai TANG E-mail: 1147067174@qq.com;tangyk@ffrc.cn

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Cite this article:

Hui LI,Wei FENG,Junjie YU,Mingyin ZHANG,Chunmiao ZHOU,Yongkai TANG. Research progress of peroxiredoxingene in crustaceans. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(3): 284-294.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.08.051 OR http://www.zjujournals.com/agr/Y2021/V47/I3/284

甲壳动物过氧化物还原酶基因的研究进展

过氧化物还原酶（peroxiredoxin, Prx）属于抗氧化蛋白超家族，广泛存在于真核生物和原核生物中，在先天免疫中发挥着重要作用。目前，有关甲壳动物Prx基因及其在先天免疫中的作用研究较少且不够深入，仅克隆出部分甲壳动物Prx基因的部分亚型，在生物学功能方面仅仅研究了其抗氧化作用。本文综述了近年来甲壳动物Prx基因的克隆、系统进化、组织表达以及生物学功能，对Prx基因在甲壳动物中的研究前景作出展望，以期为甲壳动物Prx基因结构和生物学功能研究提供参考。

关键词： 甲壳动物, 过氧化物还原酶, 基因克隆, 组织表达, 生物学功能

Table 1 Prx classification based on sequence homology and structural similarity

Table 2 Prx genes and their coding amino acid sequences of crustacean

物种 Species	Prx亚型 Prx subtype	亚细胞定位 Subcellular localization
脊尾白虾 Exopalaemon carinicauda	Prx5	过氧化物酶体（55.6%）、细胞核（22.2%）、细胞质（11.1%）、线粒体（11.1%）
中国对虾 Fenneropenaeus chinensis	Prx	细胞质（60.9%）、细胞核（21.7%）、线粒体（13.0%）、分泌系统囊泡（4.4%）
日本沼虾 Macrobrachium nipponense	Prx	细胞质（47.8%）、线粒体（26.1%）、细胞核（21.7%）、分泌系统囊泡（4.4%）
三疣梭子蟹 Portunus trituberculatus	Prx	细胞质（52.4%）、线粒体（21.7%）、细胞核（13.0%）、过氧化物酶体（4.3%）、内质网（4.3%）、质膜（4.3%）
斑节对虾 Penaeus monodon	Prx1	细胞质（47.9%）、细胞核（30.4%）、线粒体（21.7%）
	Prx4	线粒体（39.2%）、细胞质（17.4%）、内质网（17.4%）、细胞核（13.0%）、空泡（8.7%）、细胞外（4.3%）
	Prx5	线粒体（73.9%）、细胞核（17.4%）、细胞质（8.7%）
	Prxn	细胞质（47.8%）、细胞核（30.4%）、线粒体（17.4%）、高尔基体（4.4%）
中华绒螯蟹 Eriocheir sinensis	Prx	细胞质（52.3%）、线粒体（26.1%）、细胞核（13.0%）、细胞外（4.3%）、空泡（4.3%）
	Prx3	线粒体（91.4%）、细胞质（4.3%）、细胞核（4.3%）
	Prx4	线粒体（43.8%）、细胞外（13.0%）、内质网（13.0%）、细胞质（13.0%）、过氧化物酶体（4.3%）、高尔基体（4.3%）、空泡（4.3%）、细胞核（4.3%）
	Prx5	过氧化物酶体（77.8%）、线粒体（11.1%）、细胞质（11.1%）
	Prx6	细胞质（61.3%）、细胞核（30.4%）、线粒体（4.3%）、高尔基体（4%）
日本对虾 Penaeus japonicus	Prx	细胞质（47.8%）、线粒体（26.1%）、细胞核（21.7%）、分泌系统囊泡（4.4%）
	Prx3	线粒体（82.6%）、细胞核（8.7%）、细胞质（8.7%）
	Prx4	线粒体（34.8%）、细胞质（30.6%）、细胞核（8.7%）、高尔基体（8.7%）、细胞外（4.3%）、内质网（4.3%）、分泌系统囊泡（4.3%）、空泡（4.3%）
	Prx6	细胞质（56.6%）、细胞核（34.8%）、线粒体（4.3%）、高尔基体（4.3%）
印度明对虾 Fenneropenaeus indicus	Prx	细胞质（47.9%）、细胞核（30.4%）、线粒体（21.7%）
平背泥蟹 Eurypanopeus depressus	Prx	细胞质（47.8%）、线粒体（26.3%）、细胞核（13.0%）、空泡（4.3%）、内质网（4.3%）、细胞外（4.3%）
南美白对虾 Penaeus vannamei	Prx	细胞质（43.5%）、细胞核（30.4%）、线粒体（26.1%）
拟穴青蟹 Scylla paramamosain	Prx1/2	细胞质（52.2%）、细胞核（17.4%）、线粒体（17.4%）、过氧化物酶体（8.7%）、内质网（4.3%）
	Prx3	线粒体（87.0%）、细胞质（8.7%）、细胞核（4.3%）
	Prx4	线粒体（34.8%）、内质网（17.5%）、细胞质（13.0%）、细胞核（13.0%）、细胞外（8.7%）、高尔基体（8.7%）、空泡（4.3%）
	Prx5-1	过氧化物酶体（77.8%）、线粒体（11.1%）、细胞质（11.1%）
	Prx5-2	过氧化物酶体（77.8%）、线粒体（11.1%）、细胞质（11.1%）
	Prx6	细胞质（60.9%）、细胞核（30.5%）、高尔基体（4.3%）、线粒体（4.3%）
中华卤虫 Artemia sinica	Prx	细胞质（43.6%）、线粒体（30.4%）、细胞核（21.7%）、分泌系统囊泡（4.3%）
美国大盐湖卤虫 Artemia franciscana	Prx	细胞质（43.5%）、细胞核（30.4%）、线粒体（26.1%）

Table 3 Subcellular localization analysis

Fig. 1 Phylogenetic tree based on the protein sequences of Prx genes in nine animals

Table 4 Summary of biological function research of crustacean Prx


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