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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2020, Vol. 46 Issue (1): 38-46    DOI: 10.3785/j.issn.1008-9209.2019.07.221
Research articles     
Effect of flavonoid compound preservatives on the postharvest quality of banana
Jun ZENG1,2,Jiali YANG1,2,Bao YANG1,Hong ZHU1()
1.South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2.University of Chinese Academy of Sciences, Beijing 100049, China
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Abstract  

Harvested banana fruit was treated by a mixed solution of morin and luteolin (in a mass ratio of 1∶2), and the combined effect on quality maintenance and shelf life of banana was investigated. The results showed that the combined treatment significantly delayed the ripening process, effectively reduced crown rot, maintained the appearance including color and firmness, reduced the respiratory rate and delayed the emergence of ethylene release and respiratory peak. The data from nuclear magnetic resonance (NMR) spectroscopy of the peel revealed the composition and level of metabolites during the banana ripening and senescence. Principal component analysis reflected that the first principal components were carbohydrates, free amino acids and other organic components such as ethanol. In a word, the combined treatment of morin and luteolin effectively delays the changes of metabolite profiles, thus maintains the fruit quality and delays senescence. As flavonoids are natural plant metabolites, which are safe, non-toxic and cost-effective, so they have the potential to be developed into broad-spectrum fruit preservatives.

Key words： banana      morin      luteolin      nuclear magnetic resonance spectroscopy     
Received: 22 July 2019      Published: 25 February 2020
CLC:  S 668.1  
Corresponding Authors: Hong ZHU     E-mail: zhuhong@scbg.ac.cn
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Jun ZENG
Jiali YANG
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Cite this article:

Jun ZENG,Jiali YANG,Bao YANG,Hong ZHU. Effect of flavonoid compound preservatives on the postharvest quality of banana. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(1): 38-46.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.07.221     OR     http://www.zjujournals.com/agr/Y2020/V46/I1/38


黄酮类复配保鲜剂对香蕉采后品质的影响

采用按质量比1∶2比例复配的2种黄酮类化合物（桑色素-木犀草素）溶液浸泡香蕉，研究该复配保鲜剂对香蕉采后品质的影响。结果表明，桑色素-木犀草素复配处理能显著延缓香蕉的后熟进程，并有效控制香蕉轴腐病的发生。各项生理指标测定结果表明，黄酮类复配保鲜剂能较好地保持香蕉的色泽、硬度等外观品质，而且很大程度降低了香蕉果实在贮藏前期的呼吸强度，并推迟乙烯释放峰值和呼吸高峰的出现。对处理后香蕉果皮的代谢物进行核磁共振波谱分析发现，代谢物的组成及含量随着果实成熟衰老而发生明显变化。主成分分析结果表明，第1主成分为碳水化合物、游离氨基酸及乙醇等有机小分子物质。总之，桑色素-木犀草素复配处理能有效延缓代谢物成分的变化，从而保持香蕉的采后品质，延缓香蕉的成熟衰老。由于黄酮类物质属于天然的植物代谢产物，安全无毒且使用量低，因此具备开发成广谱的水果保鲜剂的潜力。


关键词： 香蕉,  桑色素,  木犀草素,  核磁共振波谱 

处理

Treatment

病情指数 Disease index
0 d10 d17 d26 d
清水 Water (CK)0a42.69±10.58a78.75±6.35a91.67±14.43a
桑色素-木犀草素 Morin-luteolin0a38.36±17.22a66.04±3.77b89.81±3.21a
Table 1 Effect of morin-luteolin treatment on controlling crown rot of banana during storage
Fig. 1 Effect of morin-luteolin treatment on the color of peel and firmness of banana fruit during storageSingle asterisk (*) indicates significant differences at the 0.05 probability level, and double asterisks (**) indicate highly significant differences at the 0.01 probability level; n=3.
Fig. 2 Effect of morin-luteolin treatment on the respiratory rate and ethylene release rate of banana fruit during storageSingle asterisk (*) indicates significant differences at the 0.05 probability level, and double asterisks (**) indicate highly significant differences at the 0.01 probability level; n=3.

编号

Nos.

代谢物

Metabolites

质子信号归属

Assignment of proton signals

1乙醇 Ethanol1.18 (t, J=7.0 Hz), 3.62 (m)
2丙氨酸 Alanine1.46 (d, J=7.3 Hz), 3.64 (m)
3乙酸 Acetic acid1.91 (s)
4谷氨酰胺 Glutamine2.12 (m), 2.47 (m), 3.66 (m)
5苹果酸 Malic acid2.45 (m), 2.75 (m), 4.27 (m)
6

L-天冬氨酸

L-aspartic acid

2.57 (m), 2.80 (m), 3.78 (m)
7胆碱 Choline3.17 (s)
8

磷酸胆碱

Choline phosphate

3.23 (s)
9果糖 Fructose4.10 (d, J=8.3 Hz)
10β-葡萄糖 β-glucose4.47 (d, J=7.8 Hz), 3.13 (m)
11α-葡萄糖 α-glucose5.11 (d, J=3.6 Hz)
12蔗糖 Sucrose

5.39 (d, J=3.8 Hz), 3.84 (m),

3.74 (m), 3.47 (m), 3.41 (m)

13

去甲猪毛菜碱

Salsolinol

6.58 (s), 6.64 (s),

1.56 (d, J=6.9 Hz)
Table 2 Assignment of proton signals in 1H-NMR mass spectra
Fig. 3 1H-NMR mass spectra of banana peel extractsPlease see the Table 2 for the details of the Nos. in Fig. 3.
Fig. 4 1H-NMR mass spectra of banana peel extracts from three storage stages
Fig. 5 PCA results for the metabolite profile of banana peel extractsA. Diagram of model parameters; B. Score plots of PCA; C. Loading plots of PCA.
[1]   姚新生.天然药物化学.2版.北京:人民卫生出版社,1996:191-195.
YAO X S. Medicinal Chemistry of Natural Products. 2nd ed. Beijing: People’s Medical Publishing House, 1996:191-195. (in Chinese)
[2]   刈米达夫.植物化学.北京:科学出版社,1985:191-195.
TATSUO K. Phytochemistry. Beijing: Science Press, 1985:191-195. (in Chinese)
[3]   MORIMOTO M, TANIMOTO K, NAKANO S, et al. Insect antifeedant activity of flavones and chromones against Spodoptera litura. Journal of Agricultural & Food Chemistry, 2003,51(2):389-393. DOI:10.1021/jf025627a
doi: 10.1021/jf025627a
[4]   ZHAO R, LI Q W, LONG L, et al. Antidiabetic activity of flavone from Ipomoea batatas leaf in non-insulin dependent diabetic rats. International Journal of Food Science & Tech-nology, 2007,42(1):80-85. DOI:10.1111/j.1365-2621.2006.01215.x
doi: 10.1111/j.1365-2621.2006.01215.x
[5]   曹纬国,刘志勤,邵云,等.黄酮类化合物药理作用的研究进展.西北植物学报,2003,23(12):2241-2247. DOI:10.13845/j.cnki.issn1673-2995.2018.03.025
CAO W G, LIU Z Q, SHAO Y, et al. A progress in pharmacological research of flavonoids. Acta Botanica Boreali-Occidentalia Sinica, 2003,23(12):2241-2247. (in Chinese with English abstract)
doi: 10.13845/j.cnki.issn1673-2995.2018.03.025
[6]   KIM Y W, HACKETT J C, BRUEGGEMEIER R W. Synthesis and aromatase inhibitory activity of novel pyridine-containing isoflavones. Journal of Medicinal Chemistry, 2004,47(16):4032-4040. DOI:10.1021/jm0306024
doi: 10.1021/jm0306024
[7]   JUNG H J, KIM S J, SONG Y S, et al. Evaluation of the antiangiogenic, anti-inflammatory, and antinociceptive activities of morin. Planta Medica, 2010,76(3):273-275. DOI:10.1055/s-0029-1186079
doi: 10.1055/s-0029-1186079
[8]   BUSTOS P S, DEZA-PONZIO R, PáEZ P L, et al. Flavonoids as protective agents against oxidative stress induced by gentamicin in systemic circulation. Potent protective activity and microbial synergism of luteolin. Food and Chemical Toxicology, 2018,118:294-302. DOI:10.1016/j.fct.2018.05.030
doi: 10.1016/j.fct.2018.05.030
[9]   AN F, WANG S L, YUAN D H, et al. Attenuation of oxidative stress of erythrocytes by plant-derived flavonoids, orientin and luteolin. Evidence-Based Complementary and Alternative Medicine, 2016,2016:3401269. DOI:10.1155/2016/3401269
doi: 10.1155/2016/3401269
[10]   ZHANG R L, LUO W D, BI T N, et al. Evaluation of antioxidant and immunity-enhancing activities of Sargassum pallidum aqueous extract in gastric cancer rats. Molecules, 2012,17(7):8419-8429. DOI:10.3390/molecules17078419
doi: 10.3390/molecules17078419
[11]   YANG S C, CHEN P J, CHANG S H, et al. Luteolin attenuates neutrophilic oxidative stress and inflammatory arthritis by inhibiting Raf1 activity. Biochemical Pharma-cology, 2018,154:384-396. DOI:10.1016/j.bcp.2018.06.003
doi: 10.1016/j.bcp.2018.06.003
[12]   ZANG M D, HU L, FAN Z Y, et al. Luteolin suppresses gastric cancer progression by reversing epithelial-mesenchymal transition via suppression of the Notch signaling pathway. Journal of Translational Medicine, 2017,15:52. DOI:10.1186/s12967-017-1151-6
doi: 10.1186/s12967-017-1151-6
[13]   YANG J L, ZENG J, WEN L R, et al. Effect of morin on the degradation of water-soluble polysaccharides in banana during softening. Food Chemistry, 2019,287:346-353. DOI:10.1016/j.foodchem.2019.02.100
doi: 10.1016/j.foodchem.2019.02.100
[14]   ZHU H, YANG J L, JIANG Y M, et al. Morin as a preservative for delaying senescence of banana. Biomolecules, 2018,8(3):biom8030052. DOI:10.3390/biom8030052
doi: 10.3390/biom8030052
[15]   秦碧霞,蔡健和,黄红,等.施保克(Sportak)浓乳剂防治香蕉采后病害药效试验.广西植保,1996,9(3):36-37.
QIN B X, CAI J H, HUANG H, et al. Experimental study on prevention and treatment of postharvest disease of banana with Sportak concentrated emulsion. Guangxi Plant Protection, 1996,9(3):36-37. (in Chinese)
[16]   刘亭,钱政江,屈红霞,等.利用LI-6262 CO2/H2O分析仪测定果蔬的呼吸强度.保鲜与加工,2010,10(4):55-56.
LIU T, QIAN Z J, QU H X, et al. Measurements of respiration intensity of harvested fruits and vegetables with LI-6262 CO2/H2O analyzer. Storage and Process, 2010,10(4):55-56. (in Chinese)
[17]   CHENG G P, YANG E, LU W J, et al. Effect of nitric oxide on ethylene synthesis and softening of banana fruit slice during ripening. Journal of Agricultural & Food Chemistry, 2009,57(13):5799-5804. DOI:10.1021/jf901173n
doi: 10.1021/jf901173n
[18]   CHOI Y H, TAPIAS E C, KIM H K, et al. Metabolic discrimination of Catharanthus roseus leaves infected by phytoplasma using 1H-NMR spectroscopy and multivariate data analysis. Plant Physiology, 2004,135(4):2398-2410. DOI:10.1104/pp.104.041012
doi: 10.1104/pp.104.041012
[19]   YUAN Y F, ZHAO Y P, YANG J L, et al. Metabolomic analyses of banana during postharvest senescence by 1H-high resolution-NMR. Food Chemistry, 2017,218:406-412. DOI:10.1016/j.foodchem.2016.09.080
doi: 10.1016/j.foodchem.2016.09.080
[20]   WEN L R, WU D, JIANG Y M, et al. Identification of flavonoids in litchi (Litchi chinensis Sonn.) leaf and evaluation of anticancer activities. Journal of Functional Foods, 2014,6(1):555-563. DOI:10.1016/j.jff.2013.11.022
doi: 10.1016/j.jff.2013.11.022
[21]   LII C Y, CHANG S M, YOUNG Y L. Investigation of the physical and chemical properties of banana starches. Journal of Food Science, 2010,47(5):1493-1497. DOI:10.1111/j.1365-2621.1982.tb04968.x
doi: 10.1111/j.1365-2621.1982.tb04968.x
[22]   ZHAO J, SHI L, ZHANG L R. Neuroprotective effect of carnosine against salsolinol-induced Parkinson’s disease. Experimental and Therapeutic Medicine, 2017,14(1):664-670. DOI:10.3892/etm.2017.4571
doi: 10.3892/etm.2017.4571
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