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| Effects and mechanism of paclobutrazol on drought resistance of Phyllostachys edulis seedlings |
| SHAO Shanlu, YANG Lizhi, TAO Chenyue, HE Anguo, YING Yeqing* |
(State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou 311300, China)
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Abstract To reveal the mechanism of paclobutrazol (PP333) on drought resistance of Phyllostachys edulis seedlings, we set the PP333 concentrations with 0 and 40 mg/L and the relative water contents with 30% (drought stress) and 70% (normal water supply), and a total of four treatments in pot experiments. Membrane lipid peroxidases and the activities of antioxidant enzymes and contents of osmotic adjustment substances were determined at the 7th, 14th and 21st days under the drought stress, and the determination of height increment of seedlings, total biomass and leaf relative water content were conducted at the 21st day under the drought stress. The results showed that compared with the normal water supply, drought stress significantly reduced the height increment, total biomass, leaf relative water content, and the activities of catalase (CAT) and peroxidase (POD), but significantly increased the root shoot ratio, malondialdehyde (MDA) content, proline (Pro) content and superoxide dismutase (SOD) activity of P. edulis seedlings. Under the drought stress, the application of PP333 significantly reduced the seedling height increment to the lowest value of 1.50 cm, while the root shoot ratio reached the maximum value of 1.35. The application of PP333 could significantly reduced MDA content and improved the leaf relative water content, proline content, and the activities of SOD, POD and CAT. Under the normal water supply condition, the effect of PP333 was similar to that under the drought stress, while its efficacy was poor than that of the latter. In conclusion, the application of PP333 can increase the relative water content, root shoot ratio, osmotic adjustment substance (Pro) content and antioxidant enzyme activities, and decrease the MDA content to improve the drought resistance of P. eduli seedlings.
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Published: 30 October 2018
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多效唑对毛竹实生苗抗旱性的影响及机制
为探讨多效唑对毛竹抗旱性的影响，进行了多效唑质量浓度为0 和40 mg/L、基质相对含水量为30%（干旱胁迫）和70%（正常供水），共4 个处理的毛竹实生苗盆栽试验。在干旱胁迫的第7 天、14 天和21 天测定各处理的实生苗膜脂过氧化酶和抗氧化酶活性及渗透调节物质含量，在干旱胁迫第21 天测定苗高增量、生物量和叶片相对含水量。结果表明：相对于正常供水，干旱胁迫显著降低了毛竹实生苗的苗高增量、总生物量、叶片相对含水量及过氧化氢酶（catalase, CAT）和过氧化物酶（peroxidase, POD）活性，显著提高了根冠比、丙二醛（malondialdehyde, MDA）、脯氨酸（Pro）含量和超氧化物歧化酶（superoxide dismutase, SOD）活性。在干旱胁迫下，施加多效唑显著降低了苗高增量，使其达到最低值1.50 cm，而根冠比达到最大值1.35；多效唑处理可显著提高叶片相对含水量、脯氨酸含量及SOD、POD、CAT活性，显著降低MDA含量。在正常供水条件下，多效唑处理的效应与干旱胁迫下的作用类似，但作用强度不及后者。综上可见，在干旱胁迫下，多效唑处理通过提高毛竹实生苗的根冠比、叶片相对含水量、脯氨酸含量和抗氧化酶活性，降低总生物量和叶片丙二醛含量而增强其抗旱性。
关键词：
干旱胁迫,
多效唑,
毛竹实生苗,
抗旱能力
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