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| Immunoregulatory function of Ganoderma spore oil in immunocompromised mice. Journal of Zhejiang University |
| YI Youjin1,2,3#, HU shun1,4#, XIONG Xingyao2*, LIU Dongbo2, ZHONG Yingli2, YI Chuanzhu5 |
| 1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop, Hunan Agricultural University, Changsha 410128, China; 3. Hunan Province Key Laboratory of Food Science and Biotechnology, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 4. Preventive Medicine and Department of Medical Laboratory, Xiangnan Institute, Chenzhou, Hunan 423000, China; 5. Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China |
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Abstract Ganoderma lucidum as a Chinese traditional medicine has been used as a medicine and food for a very long time. Ganoderma spore oil, a sort of lipin extracted from Ganoderma spore (the reproductive organs of Ganoderma lucidum), is rich in ganodenic acid, unsaturated fatty acid, Reishi polysaccharides and so forth. The reports on immunoregulation and antioxidation of Ganoderma spore oil are few at present. Cyclophosphamide (CPA) is a common antineoplastic agent which can cause immunodepression. In order to reduce clinically immunocompromised side effects caused by CPA, the immunedeficient mouse model was established to study the immunoregulation effect of Ganoderma spore oil on immunocompromised mice.
Ganoderma spore oil was given to hypoimmune model induced by injecting Kunming mice with CPA. The mice were randomly divided into nomal control group, model control group, Ganoderma spore oil group. Ganoderma spore oil group accepted 0.01 mL/g of 150 mg/kg Ganoderma spore oil for 30 d. Normal control group and model control group were given corn oil of 0.01 mL/g for 30 d. Model control group and Ganoderma spore oil group were given 40 mg/kg CPA once a day for 1, 3, 5, 17, 19, 21 d respectively, to establish immunedeficient mouse model. Nomal control group was injected subcutaneously with normal saline. The spleen and thymus index, lymphocyte transformation and delayedtype hypersensitivity (DTH), hemolysin antibody level and hemolysin content, natural killer (NK) cell activity were measured at the 30th day.
The results showed that compared with normal control group, the measured parameters were low for model control group. However, the related parameters for Ganoderma spore oil group were higher than model control group and the difference was obvious (P<0.05), indicating that the Ganoderma spore oil could increase the spleen index, thymus index, lymphocyte transformation and DTH level, and improve the content of serum hemolysin in immunocompromised mice. In addition, the Ganoderma spore oil had remarkable promoting effect on immune regulation of immunocompromised mice, including cell immunity, humoral immunity and nonspecific immunity.
These results indicate Ganoderma spore oil can stimulate both specific and nonspecific immune function in mice, and enhance the immunoregulation functions of the immunocompromised mice according to the Chinese Theory Evaluating Procedure and Test Method of Health Foods.
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Published: 20 March 2013
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Cite this article:
YI Youjin1,2,3#, HU shun1,4#, XIONG Xingyao2*, LIU Dongbo2, ZHONG Yingli2, YI Chuanzhu5. Immunoregulatory function of Ganoderma spore oil in immunocompromised mice. Journal of Zhejiang University. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(2): 161-166.
URL:
http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.11.067 OR http://www.zjujournals.com/agr/Y2013/V39/I2/161
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灵芝孢子油对免疫低下模型小鼠的免疫调节作用
通过给昆明种小鼠隔天皮下注射环磷酰胺建立免疫功能低下模型，用灵芝孢子油灌胃小鼠30 d，分别测定小鼠的免疫器官指数、脾淋巴细胞转化率和迟发型变态反应、抗体生成细胞数和血清溶血素水平、自然杀伤细胞活性，以研究灵芝孢子油对小鼠免疫功能的调节作用. 结果表明：灵芝孢子油能显著提高免疫低下模型小鼠的脾脏指数(P<0.05)和胸腺指数(P<0.05)；提高小鼠脾淋巴细胞转化率和迟发型变态反应水平(P<0.05)；增强血清溶血素水平和抗体生成细胞数(P<0.05)；改善小鼠自然杀伤细胞的活性(P<0.05). 说明灵芝孢子油能提高免疫低下小鼠的体液免疫、细胞免疫和非特异性免疫功能，对机体特异性及非特异性免疫功能均具有显著调节作用.
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