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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2013, Vol. 39 Issue (3): 246-252    DOI: 10.3785/j.issn.1008-9209.2012.10.061
    
Cloning and sequence analysis of three novel chalone synthase genes in Cryptomeria plants
LU Yongquan1*, JIA Qing1, TONG  Zaikang1， CHEN  Jianyang2
（1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin′an, Zhejiang 311300, China； 2. Tiantai County Huading Forestry Farm, Tiantai, Zhejiang  317200, China)
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Abstract  Chalcone synthase (CHS) is the first enzyme in the flavonoid biosynthetic pathway in plants. In order to clone CHS gene in Cryptomeria plants, the amplified consensus genetic markers (ACGM) were designed and combined with RACE technique, three CHS genes in Cryptomeria japonica var. sinensis，C. japonica cv. Araucarioides and C. japonica were obtained, respectively. Sequence analysis showed that the coding sequence (CDS) of each CHS gene was 1176 bp in length and encoded 391 amino acids. The GFGPG tag of CHS conservative active sites were found in their proteins. Three CHS proteins showed 99% homology with each other, but more than 79% homology with other plants, which indicated that CHS were  relatively conserved during evolution.

Published: 20 May 2013
CLC:  Q 74  
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TONG Zaikang1
CHEN Jianyang2
LU Yongquan1*
JIA Qing1
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LU Yongquan1*, JIA Qing1, TONG Zaikang1， CHEN Jianyang2. Cloning and sequence analysis of three novel chalone synthase genes in Cryptomeria plants. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(3): 246-252.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.10.061     OR     http://www.zjujournals.com/agr/Y2013/V39/I3/246


柳杉属3个查尔酮合成酶（CHS）新基因的克隆及其序列特异性分析(英文)

查尔酮合成酶（chalcone synthase， CHS)是黄酮类物质合成的第一关键酶。为了获得CHS基因，我们在 ACGM标记所扩增的PCR片段内设计锚定引物，并结合RACE技术首次克隆了柳杉属3种植物的CHS基因，包括柳杉（Cryptomeria japonica var. sinensis， CjsCHS）、短茸柳杉（C. japonica cv. Araucarioides，CjaCHS）和日本柳杉（C. japonica，CjCHS），并对其进行分析。结果表明:3个基因长都为1176 bp，编码391个氨基酸，它们都含有CHS高度保守活性位点以及CHS标签序列GFGPG，其编码蛋白的相似度达99%,与其他植物相似度在 79%以上,表明CHS基因在进化上具有相对保守性。
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