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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (4): 387-392    DOI: 10.3785/j.issn.1008-9209.2011.04.005
Biological sciences & biotechnology     
Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber
JING Ran１ , CHEN Xin-juan２ , ZHU Yu-qiang２ , ZHANG Peng２ , ZHANG Yu-qiong１ , ZHOU Shengjun２
1 . School of Life Science , Anhui Agricultural University , Hefei 230036 , China ;2 . Institute of Vegetables , Zhejiang Academy of Agricultural Sciences , Hangzhou 310021 , China
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Abstract  With a F２ population between a resistant parent and susceptible parent , SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber . Sixty‐two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from ４３７ pairs of SRAP primer combinations . Further analyzing the F２ segregating populations using these primers , one SRAP marker of Me1 /Em9 — 284 bp linked to the powdery mildew resistance was found , with 9.8 cM genetic distance . The marker can be used for cloning disease‐resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection .

Published: 20 July 2011
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CHEN Xin-juan
ZHANG Yu-qiong
JING Ran
ZHU Yu-qiang
ZHANG Peng
ZHOU Sheng-jun
Cite this article:

JING Ran,CHEN Xin-juan,ZHU Yu-qiang,ZHANG Peng,ZHANG Yu-qiong,ZHOU Sheng-jun. Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 387-392.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.04.005     OR     http://www.zjujournals.com/agr/Y2011/V37/I4/387


黄瓜白粉病抗性序列相关扩增多态性的分子标记研究

以高抗和高感白粉病的黄瓜亲本及其杂交后代F２ 分离群体为材料,应用序列相关扩增多态性(sequence-related amplified polymorphism , SRAP) 技术开展与黄瓜白粉病抗性基因连锁的SRAP 分子标记研究.结果表明:从４３７ 对SRAP 引物组合中筛选出６２ 对能够在２ 个亲本抗、感池间扩增出稳定多态性条带的引物组合,进一步用这些引物分析F２ 群体,发现有１ 对引物组合Me１/Em９ 扩增出的SRAP标记(Me1/Em9----284 bp)能与黄瓜抗白粉病基因连锁,遗传距离为９ .８ cM .获得的分子标记对克隆抗病基因和利用分子标记辅助选择提高育种效率具有重要意义.
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