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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University: Agric. & Life Sci.  2011, Vol. 37 Issue (4): 355-362    DOI: 10.3785/j.issn.1008-9209.2011.04.001
Biological sciences & biotechnology     
Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus .
ZHANG Xiao-dong1,2, , SUN Jing1, , LI Jian-rong2, , YU Lian1
1.Institute o f Preventive Veterinary Medicine , College o f A nimal Sciences , Zhe j iang University , H angz hou 310029 , China ;2 . Dep artment o f Food Science and Technology , The Ohio State University , Columbus , O H 43210 ,USA
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Abstract  To prevent the vesicular stomatitis virus ( VSV) disease , using a panel of recombinant VSV (E１７６４A , S１８２７A , Y１６５０A , and F１６９１A) that were defective in mRNA cap methylation as vaccine to inoculate five‐week‐old BALB/c female mice through oral route with １ × １０６ PFU , the pathogenicity and immunogenicity of these viruses were determined . The result showed that recombinant S １８２７A , Y１６５０A , F１６９１A were attenuated in mice and E１７６４A was still pathogenic to mice . The immunized mice were challenged with wild type VSV . Mice immunized with S１８２７A and Y１６５０A trigged a high level of neutralizing antibody and were protected from virulent challenge . Taken together , the results above demonstrated that methyltransferase ( MTase)‐defective VSV ( S１８２７A and Y１６５０A) were not only attenuated in animals , but also exhibited excellent immunogenicity . Therefore , MTase‐defective viruses will be good live vaccine candidates against VSV .

Published: 20 July 2011
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ZHANG Xiao-dong,SUN Jing,LI Jian-rong,YU Lian. Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus .. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 355-362.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.04.001     OR     http://www.zjujournals.com/agr/Y2011/V37/I4/355


重组甲基转移酶致弱水疱性口炎病毒的致病性与免疫原性

为了研究有效的水疱性口炎病毒疫苗,控制水疱性口炎疾病的蔓延,用甲基化能力致弱的重组水疱性口炎病毒VSV‐E１７６４A ,VSV‐S１８２７A ,VSV‐Y１６５０A 和VSV‐F１６９１A 作为疫苗,通过口腔灌服途径将１ × １０６ PFU 的重组病毒接种５ 周龄BALB/c 小鼠,研究以上重组病毒的致病性和免疫原性.结果表明:重组病毒VSV‐S１８２７A ,VSV‐Y１６５０A 和VSV‐F１６９１A 对小鼠的致病性减弱,而VSV‐E１７６４A仍然具有较强的致病性;免疫后的小鼠用野生型水疱性口炎病毒( VSV) 进行攻毒,发现VSV‐S１８２７A和VSV‐Y１６５０A 能够诱导高水平的中和抗体,从而保护小鼠免于攻击.总之,甲基化酶致弱的水疱性口炎病毒VSV‐S１８２７A 和VSV‐Y１６５０A 不仅对动物的致病性减弱,而且表现出良好的免疫原性;因此,甲基化酶致弱的水疱性口炎病毒可能成为有良好开发前景的活疫苗.
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