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, Volume 6 Issue 3   
Development of Pasteuria penetrans in Meloidogyne javanica females as affected by constantly high vs fluctuating temperature in an in-vivo system
DARBAN D.A., GOWEN S.R., PEMBROKE B., MAHAR A.N.
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 1-.   https://doi.org/10.1631/jzus.2005.B0155
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Growth room and glasshouse experiment was conducted to investigate the effect of constant and fluctuating temperatures on the development of Pasteuria penetrans a hyperparasite of root-knot nematodes. Tomato plants (Lycopersicon esculentum Mill) were inoculated with Meloidogyne javanica second-stage juveniles attached with endospores of P. penetrans and were grown in growth room at 26-29 ??C and in glasshouse at 20-32 ??C. The tomato plants were sampled from the growth room after 600 degree-days based on 17 ??C/d, accumulating each day above a base temperature of 10 ??C and from the glasshouse after 36 calendar days. Temperature affected the development of P. penetrans directly. The rate of development at constant temperature in growth room was faster than that in the glasshouse at fluctuating temperatures.
Molecular analysis of microbial community in a groundwater sample polluted by landfill leachate and seawater
TIAN Yang-jie, YANG Hong, WU Xiu-juan, LI Dao-tang
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 3-.   https://doi.org/10.1631/jzus.2005.B0165
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Seashore landfill aquifers are environments of special physicochemical conditions (high organic load and high salinity), and microbes in leachate-polluted aquifers play a significant role for intrinsic bioremediation. In order to characterize microbial di
Bioremediation potential of spirulina: toxicity and biosorption studies of lead
CHEN Hong, PAN Shan-shan
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 4-.   https://doi.org/10.1631/jzus.2005.B0171
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This study examines the possibility of using live spirulina to biologically remove aqueous lead of low concentration (below 50 mg/L) from wastewater. The spirulina cells were first immersed for seven days in five wastewater samples containing lead of different concentrations, and the growth rate was determined by light at wavelength of 560 nm. The 72 h-EC50 (72 h medium effective concentration) was estimated to be 11.46 mg/L (lead). Afterwards, the lead adsorption by live spirulina cells was conducted. It was observed that at the initial stage (0¨C12 min) the adsorption rate was so rapid that 74% of the metal was biologically adsorbed. The maximum biosorption capacity of live spirulina was estimated to be 0.62 mg lead per 105 alga cells.
Experiment and mechanism investigation on advanced reburning for NOx reduction: influence of CO and temperature
WANG Zhi-hua, ZHOU Jun-hu, ZHANG Yan-wei, LU Zhi-min, FAN Jian-ren, CEN Ke-fa
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 7-.   https://doi.org/10.1631/jzus.2005.B0187
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Pulverized coal reburning, ammonia injection and advanced reburning in a pilot scale drop tube furnace were investigated. Premix of petroleum gas, air and NH3 were burned in a porous gas burner to generate the needed flue gas. Four kinds of pulverized coa
Study on structural characteristics of pillared clay modified phosphate fertilizers and its increase efficiency mechanism
WU Ping-xiao, LIAO Zong-wen
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 8-.   https://doi.org/10.1631/jzus.2005.B0195
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Three types of new high-efficiency phosphate fertilizers were made when pillared clays at certain proportions were added into ground phosphate rock. Chemical analyses showed that their soluble phosphorus content decreased more than that of superphosphate. Pot experiment showed that, under equal weights, the new fertilizers increased their efficiency by a large margin over that of superphosphate. Researches on their structures by means of XRD, IR and EPR spectrum revealed that their crystal structures changed considerably, improving their activity and preventing the fixation of available phosphorus in the soil, and consequently, greatly improved the bioavailability and became the main cause of the increase of biomass.
Hydrogenation properties of mechanically milled Mg2Ni0.8Cr0.2-CoO/Al2O3 composites
WANG Xiu-li, TU Jiang-ping, CHEN Chang-pin, ZHANG Xiao-bin, ZHAO Xin-bing
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 10-.   https://doi.org/10.1631/jzus.2005.B0208
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Mg2Ni0.8Cr0.2-x wt.% CoO/Al2O3 (x=0.5, 1, 2 and 3) composites were prepared by mechanically milling sintered Mg2Ni0.8Cr0.2 alloy and CoO/Al2O3 compound for 45 h. The addition of CoO/Al2O3 compound resulted in the good kinetics properties of hydriding/dehydriding reaction of the composites. The composite with 1.0 wt.% CoO/Al2O3 catalyst could reach the maximum hydrogen absorption capacity (2.9 wt.%) within 5 min at 393 K under H2 pressure of 4 MPa, and can desorb rapidly at 493 K. The decomposition and synthesis of hydrogen molecule on Mg2Ni0.8Cr0.2 alloy surface was promoted by addition of CoO/Al2O3 catalyst. In addition, the formation of metallic Ni particles, strain and defects during the ball milling process also resulted in the improved hydrogenation performance of Mg2Ni-based alloys.
Effect of polyoxypropylene chain length on the critical micelle concentration of propylene oxide-ethylene oxide block copolymers
ZHANG Zhi-guo, YIN Hong
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 3): 12-.   https://doi.org/10.1631/jzus.2005.B0219
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In this work, the surface activity of block copolymer nonionic surfactants (RPE) has been determined, i.e., critical micelle concentration (CMC), surface excess concentration (|£), surface area demand per molecule (A), surface tension at CMC (|?CMC). A linear decrease of ln[CMC] vs number of oxypropylene units in copolymer molecule was observed. The change in the work of cohesion per oxypropylene group when passing from molecular into micellar state, calculated from the Shinoda equation, was 0.43kT for the studied compounds.
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