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| In vitro simulation studies of silica deposition induced by lignin from rice |
| Fang Jiang-Yu, Ma Xue-Long |
| Institute of Biodiversity Research, Huangshan University, Huangshan 245021, China; Tea Biochemistry and Biotechnology Laboratory of Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China |
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Abstract To reveal the possible mechanism of silica deposition in higher plants, lignin was isolated from rice straw following a modified method to conduct a simulation experiment in vitro. UV and infrared absorption spectra showed that the substance had the unique characteristics of pure lignin. The presence of silicon in the precipitation was revealed by TEM (transmission electron microscopy) with EDXA (energy dispersive X-ray analysis) device. It was found that in the borax solution where lignin precipitation occurred silica-lignin co-precipitation was produced but not in the DMSO solution where lignin was broken into its composition compounds and did not precipitate. This means that it is macromolecular lignin itself but not its compounds that could induce silica deposition in higher plants.
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Received: 12 December 2005
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