Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2005, Vol. 6 Issue ( 5): 16-    DOI: 10.1631/jzus.2005.B0396
    
Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy
XU Zhi-nan, SHEN Wen-he, CHEN Hao, CEN Pei-lin
Institute Bioengineering, School of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  Plasmid vector is increasingly applied to gene therapy or gene vaccine. The production of plasmid pCMV-AP3 for cancer gene therapy was conducted in a modified MBL medium using a recombinant E. coli BL21 system. The effects of different MMBL comp

Key words： Bioengineering Material Science and Chemical Engineering Plasmid DNA      Growth medium      Gene therapy     
Received: 10 February 2004     
CLC:  Q782  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
XU Zhi-nan
SHEN Wen-he
CHEN Hao
CEN Pei-lin
Cite this article:

XU Zhi-nan, SHEN Wen-he, CHEN Hao, CEN Pei-lin. Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 5): 16-.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2005.B0396     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2005/V6/I 5/16

[1] Xu-wei Luo, Kang Liu, Zhu Chen, Ming Zhao, Xiao-wei Han, Yi-guang Bai, Gang Feng. Adenovirus-mediated GDF-5 promotes the extracellular matrix expression in degenerative nucleus pulposus cells[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(1): 30-42.
[2] Qi-da Hu, Hui Fan, Wei-jian Lou, Qing-qing Wang, Gu-ping Tang. Polyethylenimine-cyclodextrin-tegafur conjugate shows anti-cancer activity and a potential for gene delivery[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(9): 720-729.
[3] Yong-song GUAN, Yuan LIU, Qing ZOU, Qing HE, Zi LA, Lin YANG, Ying HU. Adenovirus-mediated wild-type p53 gene transfer in combination with bronchial arterial infusion for treatment of advanced non-small-cell lung cancer, one year follow-up[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2009, 10(5): 331-340.
[4] Sun Huai-Chang, Xue Fang-Ming, Qian Ke, Fang Hao-Xia, Qiu Hua-Lei, Zhang Xin-Yu, Yin Zhao-Hua. Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(4 ): 13-.
[5] LI Da, WANG Qing-qing, TANG Gu-ping, HUANG Hong-liang, SHEN Fen-ping, LI Jing-zhong, YU Hai. Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(11): 10-.