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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2015, Vol. 16 Issue (10): 865-874    DOI: 10.1631/jzus.B1500029
Articles     
Isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental Ochrobactrum intermedium isolate versus clinical strains
Lu-jun Chai, Xia-wei Jiang, Fan Zhang, Bei-wen Zheng, Fu-chang Shu, Zheng-liang Wang, Qing-feng Cui, Han-ping Dong, Zhong-zhi Zhang, Du-jie Hou, Yue-hui She
1Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, School of Energy Resources, China University of Geosciences, Beijing 100083, China; 2State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; 3College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China; 4Institute of Porous Flow & Fluid Mechanics, Chinese Academy of Sciences, Langfang 065007, China; 5State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 257061, China
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Abstract  In this study, we isolated an environmental clone of Ochrobactrum intermedium, strain 2745-2, from the formation water of Changqing oilfield in Shanxi, China, which can degrade crude oil. Strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °C and pH 5.5. We sequenced the genome and found a single chromosome of 4 800 175 bp, with a G+C content of 57.63%. Sixty RNAs and 4737 protein-coding genes were identified: many of the genes are responsible for the degradation, emulsification, and metabolizing of crude oil. A comparative genomic analysis with related clinical strains (M86, 229E, and LMG3301T) showed that genes involved in virulence, disease, defense, phages, prophages, transposable elements, plasmids, and antibiotic resistance are also present in strain 2745-2.

Key words： Comparative genome      Ochrobactrum intermedium      Oil degradation      Pathogen     
Received: 26 January 2015      Published: 03 October 2015
CLC:  X172  
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Lu-jun Chai
Zhong-zhi Zhang
Du-jie Hou
Yue-hui She
Xia-wei Jiang
Fan Zhang
Bei-wen Zheng
Fu-chang Shu
Zheng-liang Wang
Qing-feng Cui
Han-ping Dong
Cite this article:

Lu-jun Chai, Xia-wei Jiang, Fan Zhang, Bei-wen Zheng, Fu-chang Shu, Zheng-liang Wang, Qing-feng Cui, Han-ping Dong, Zhong-zhi Zhang, Du-jie Hou, Yue-hui She. Isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental Ochrobactrum intermedium isolate versus clinical strains. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(10): 865-874.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1500029     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I10/865

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