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| Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet |
| Sheng-ping Wang, Yun-ling Gao, Gang Liu, Dun Deng, Rong-jun Chen, Yu-zhe Zhang, Li-li Li, Qing-qi Wen, Yong-qing Hou, Ze-meng Feng, Zhao-hui Guo |
| Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Hunan Institute of Microbiology, Changsha 410009, China; Rice Research Institute of Sichuan Agricultural University, Chengdu 625014, China; Fujian Aonong Biotechnology Corporation, Xiamen 361007, China; Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China |
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Abstract The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access number is GQ414763. The pEnho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of pEnho in tissues (liver, muscle, anterior jejunum, posterior jejunum, and ileum) was determined by quantitative real-time RT-PCR. pEnho was localized on porcine chromosome 10 and no introns were found. In conclusion, pEnho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.
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Received: 26 September 2014
Published: 08 June 2015
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Cite this article:
Sheng-ping Wang, Yun-ling Gao, Gang Liu, Dun Deng, Rong-jun Chen, Yu-zhe Zhang, Li-li Li, Qing-qi Wen, Yong-qing Hou, Ze-meng Feng, Zhao-hui Guo. Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(6): 524-532.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400260 OR http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I6/524
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