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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (7): 611-623    DOI: 10.1631/jzus.B1300320
Articles     
Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.)
Chang-ying Liu, Rui-hua Lü, Jun Li, Ai-chun Zhao, Xi-ling Wang, Umuhoza Diane, Xiao-hong Wang, Chuan-hong Wang, Ya-sheng Yu, Shu-mei Han, Cheng Lu, Mao-de Yu
State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing 400715, China
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Abstract  1-Aminocyclopropane-1-carboxylic acid synthase (ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type I, MaACS3 and MaACS4 are type II, and MaACS5 is type III, with different C-terminal sequences. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demonstrated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid (ABA) and ethephon.

Key words： Mulberry fruit      MaACS      MaACO      Gene expression      Abscisic acid      Ethephon     
Received: 18 December 2013      Published: 06 July 2014
CLC:  S888.2  
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Ya-sheng Yu
Shu-mei Han
Cheng Lu
Mao-de Yu
Chang-ying Liu
Ai-chun Zhao
Rui-hua Lü
Jun Li
Xi-ling Wang
Umuhoza Diane
Xiao-hong Wang
Chuan-hong Wang
Cite this article:

Chang-ying Liu, Rui-hua Lü, Jun Li, Ai-chun Zhao, Xi-ling Wang, Umuhoza Diane, Xiao-hong Wang, Chuan-hong Wang, Ya-sheng Yu, Shu-mei Han, Cheng Lu, Mao-de Yu. Characterization and expression profiles of MaACS and MaACO genes from mulberry (Morus alba L.). Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(7): 611-623.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300320     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I7/611

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