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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (2): 153-164    DOI: 10.1631/jzus.B1300230
Articles     
Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer
Yi Lan, Qing Wu, Ying-qiu Mao, Qiong Wang, Jing An, Yan-yan Chen, Wen-ping Wang, Bo-chen Zhao, Na Liu, Ye-wen Zhang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; Center of Science Experiment, Beijing University of Chinese Medicine, Beijing 100029, China
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Abstract  The aim of this present study is to investigate the effect of Zanthoxylum bungeanum oil (essential oil from Z. bungeanum Maxim.) on cytotoxicity and the transdermal permeation of 5-fluorouracil and indomethacin. The cytotoxicity of Z. bungeanum oil on dermal fibroblasts and epidermal keratinocytes was studied using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The rat skin was employed to determine the percutaneous penetration enhancement effect of Z. bungeanum oil on hydrophilic and lipophilic model drugs, i.e., 5-fluorouracil and indomethacin. The secondary structure changes of the rat stratum corneum (SC) were determined using attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and saturated solubilities and SC/vehicle partition coefficients of two model drugs with and without Z. bungeanum oil were also measured to understand its related mechanisms of action. It was found that the half maximal inhibitory concentration (IC50) values of Z. bungeanum oil were significantly lower in HaCaT and CCC-ESF-1 cell lines compared to the well-established and standard penetration enhancer Azone. The Z. bungeanum oil at various concentrations effectively facilitated the percutaneous penetration of two model drugs across the rat skin. In addition, the mechanisms of permeation enhancement by Z. bungeanum oil could be explained with saturated solubility, SC/vehicle partition coefficient, and secondary structure changes of SC.

Key words： Zanthoxylum bungeanum Maxim.      Essential oil      Transdermal delivery      Penetration enhancer      HaCaT      Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR)     
Received: 28 August 2013      Published: 28 January 2014
CLC:  R28  
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Na Liu
Ye-wen Zhang
Yi Lan
Qiong Wang
Qing Wu
Ying-qiu Mao
Jing An
Yan-yan Chen
Wen-ping Wang
Bo-chen Zhao
Cite this article:

Yi Lan, Qing Wu, Ying-qiu Mao, Qiong Wang, Jing An, Yan-yan Chen, Wen-ping Wang, Bo-chen Zhao, Na Liu, Ye-wen Zhang. Cytotoxicity and enhancement activity of essential oil from Zanthoxylum bungeanum Maxim. as a natural transdermal penetration enhancer. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(2): 153-164.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300230     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I2/153

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