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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2014, Vol. 15 Issue (2): 143-152    DOI: 10.1631/jzus.B1300239
Articles     
Stability and cytotoxicity of angiotensin-I-converting enzyme inhibitory peptides derived from bovine casein
Wei Wu, Pan-pan Yu, Feng-yang Zhang, Hong-xia Che, Zhan-mei Jiang
Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China
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Abstract  This study investigated the effect of heat treatment combined with acid and alkali on the angiotensin-I-converting enzyme (ACE) inhibitory activity of peptides derived from bovine casein. The free amino group content, color, and cytotoxicity of the peptides were measured under different conditions. When heated at 100 °C in the pH range from 9.0 to 12.0, ACE inhibitory activity was reduced and the appearance of the peptides was significantly darkened. After thermal treatment in the presence of acid and alkali, the free amino group content of ACE inhibitory peptides decreased markedly. High temperature and prolonged heating also resulted in the loss of ACE inhibitory activity, the loss of free amino groups, and the darker coloration of bovine casein-derived peptides. However, ACE inhibitory peptides, within a concentration range of from 0.01 to 0.2 mg/ml, showed no cytotoxicity to Caco-2 and ECV-304 cell lines after heat treatment. This indicated that high temperature and alkaline heat treatment impaired the stability of bovine casein-derived ACE inhibitory peptides.

Key words： Angiotensin-I-converting enzyme inhibitory peptide      Heat treatment      Stability      Cytotoxicity     
Received: 04 September 2013      Published: 28 January 2014
CLC:  S872  
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Wei Wu
Hong-xia Che
Pan-pan Yu
Feng-yang Zhang
Zhan-mei Jiang
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Wei Wu, Pan-pan Yu, Feng-yang Zhang, Hong-xia Che, Zhan-mei Jiang. Stability and cytotoxicity of angiotensin-I-converting enzyme inhibitory peptides derived from bovine casein. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(2): 143-152.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1300239     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2014/V15/I2/143

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