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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2011, Vol. 12 Issue (2): 116-125    DOI: 10.1631/jzus.B1000278
Biotechnology     
Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection*#
Qiu-lei Lang, Xiao-chuan Zhou, Xiao-lin Zhang, Rafal Drabek, Zhi-xiang Zuo, Yong-liang Ren, Tong-bin Li, Ji-shuang Chen, Xiao-lian Gao
College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; LC Sciences, 2575 W. Bellfort, Suite 270, Houston, TX 77054, USA; Department of Biology and Biochemistry, University of Houston, Houston, TX 77004, USA
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Abstract  A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate that developmental anomalies elicited by virus infection may be caused by more complex biological processes.

Key words： Solanum lycopersicum      Plant miRNA      Cucumber mosaic virus      miRNA array      miRNA response     
Received: 21 July 2010      Published: 04 February 2011
CLC:  Q945.8  
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Qiu-lei Lang
Xiao-lian Gao
Xiao-chuan Zhou
Xiao-lin Zhang
Rafal Drabek
Zhi-xiang Zuo
Yong-liang Ren
Tong-bin Li
Ji-shuang Chen
Cite this article:

Qiu-lei Lang, Xiao-chuan Zhou, Xiao-lin Zhang, Rafal Drabek, Zhi-xiang Zuo, Yong-liang Ren, Tong-bin Li, Ji-shuang Chen, Xiao-lian Gao. Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection*#. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(2): 116-125.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000278     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2011/V12/I2/116

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