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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2011, Vol. 12 Issue (1): 55-64    DOI: 10.1631/jzus.B1000174
Biotechnology     
Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats
Wei Zhang, Quan-wei Wei, Zheng-chao Wang, Wei Ding, Wei Wang, Fang-xiong Shi
Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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Abstract  Objective: The present study is designed to investigate the cellular expressions and immunolocalizations of three different nitric oxide synthase (NOS) isoforms and the related nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) signaling pathway in the ovaries of neonatal and immature rats. Methods: The ovaries were obtained from ICR (Institute for Cancer Research) female Sprague-Dawley rats at postnatal days 1, 5, 7, 10, and 19. Then we carried out the histologic examination, immunohistochemistry, measurement of NOS activity, and modifications within the NO/cGMP pathway. Results: During postnatal days 1, 5, 7, 10, and 19, all three isoforms of NOS were mainly localized to the oocytes and expressed as a gradual increase in granulosa cells and theca cells within the growing follicle. The ovarian total NOS activities and NO levels were increased at postnatal days 7 and 10 compared with other days. Conclusions: Our findings suggest that the locally produced NO and the NO/NOS signaling systems are involved in the follicular development to puberty.

Key words： Nitric oxide synthase      Nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) pathway      Ovary      Neonatal rats      Prepuberty     
Received: 11 May 2010      Published: 06 January 2010
CLC:  Q492.5  
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Zheng-chao Wang
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Quan-wei Wei
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Fang-xiong Shi
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Wei Zhang, Quan-wei Wei, Zheng-chao Wang, Wei Ding, Wei Wang, Fang-xiong Shi. Cell-specific expression and immunolocalization of nitric oxide synthase isoforms and the related nitric oxide/cyclic GMP signaling pathway in the ovaries of neonatal and immature rats. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(1): 55-64.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000174     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2011/V12/I1/55

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