| Biotechnology and Biochemistry |
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| Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside |
| Xiao-kun WEI, Qing-bao DING, Lu ZHANG, Yong-li GUO, Lin OU, Chang-lu WANG |
| College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Weiping Bioscience and Biotechnology Co., Ltd., Shanghai 200237, China |
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Abstract Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5′-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.
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Received: 12 December 2007
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