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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2008, Vol. 9 Issue (9): 728-733    DOI: 10.1631/jzus.B0820095
Biomedicine     
Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector
Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
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Abstract  A high-performance liquid chromatographic (HPLC) method with ultraviolet (UV) detector had been developed for simultaneous quantification of danshensu, protocatechuic aldehyde, caffeic acid, salvianolic acid D, rosmarinic acid, salvianolic acid B and salvianolic acid A in Danshen injection. According to the UV spectra of these components, three detection wavelengths have been selected as follows: 280 nm for danshensu and protocatechuic aldehyde, 326 nm for caffeic acid, salvianolic acid D and rosmarinic acid, 286 nm for salvianolic acid B and salvianolic acid A. The limit of detection (LOD) was improved to be in the range of 0.008~0.160 μg/ml. Moreover, excellent linear behavior over the investigated concentration range was observed, with R>0.999 for all the analytes.

Key words： High-performance liquid chromatography (HPLC)      Danshen injection (DSI)      Phenolic acids      Multi-wavelengths      Quantification     
Received: 20 March 2008     
CLC:  R91  
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Jin-zhong XU
Jie SHEN
Yi-yu CHENG
Hai-bin QU
Cite this article:

Jin-zhong XU, Jie SHEN, Yi-yu CHENG, Hai-bin QU. Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(9): 728-733.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0820095     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2008/V9/I9/728

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