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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2007, Vol. 8 Issue (11): 839-844    DOI: 10.1631/jzus.2007.B0839
Biotechnology     
Type-dependent differential expression of neuropeptide Y in chicken hypothalamus (Gallus domesticus)
CHEN Gui-qian, HU Xiu-fang, SUGAHARA Kunio, CHEN Ji-shuang, SONG Xue-mei, ZHENG Hui-chao, JIANG Yong-qing, HUANG Xin, JIANG Jun-fang, ZHOU Wei-dong
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Laboratory of Nutritional Biochemistry, Department of Animal Science, Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan
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Abstract  Neuropeptide Y (NPY) is one of the most important orexigenic agents in central regulation of feeding behavior, body weight and energy homeostasis in domestic chickens. To examine differences in the hypothalamic NPY between layer-type and meat-type of chickens, which are two divergent kinds of the domestic chickens in feeding behavior and body weight, we detected mRNA levels of NPY in hypothalamic infundibular nucleus (IN), paraventricular nucleus (PVN) and lateral hypothalamic area (LHA) of these two types of chickens using one-step real time RT-PCR. The meat-type chicken had more food daily (about 1.7 folds) and greater body weights (about 1.5 folds) and brain weights than the layer-type chicken at the age of 14 d. In the meat-type of chicken, NPY mRNA levels of the IN and PVN were significantly greater than those of the LHA, and were not significantly different between the IN and PVN. However, in the layer-type of chicken, NPY mRNA levels were significantly greater in the IN than those in the LHA and PVN, and were not significantly different between the PVN and LHA. In all these hypothalamic regions, the layer-type of chicken had significantly higher NPY mRNA levels than the meat-type chicken did. These results suggest the expression of NPY in the hypothalamus has a type-dependent pattern in domestic chickens.

Key words： Neuropeptide Y (NPY)      Hypothalamus      Message RNA (mRNA)      Meat-type chicken      Layer-type chicken     
Received: 09 July 2007     
CLC:  Q78  
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HUANG Xin
JIANG Jun-fang
ZHOU Wei-dong
CHEN Gui-qian
HU Xiu-fang
SUGAHARA Kunio
CHEN Ji-shuang
SONG Xue-mei
ZHENG Hui-chao
JIANG Yong-qing
Cite this article:

CHEN Gui-qian, HU Xiu-fang, SUGAHARA Kunio, CHEN Ji-shuang, SONG Xue-mei, ZHENG Hui-chao, JIANG Yong-qing, HUANG Xin, JIANG Jun-fang, ZHOU Wei-dong. Type-dependent differential expression of neuropeptide Y in chicken hypothalamus (Gallus domesticus). Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(11): 839-844.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2007.B0839     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2007/V8/I11/839

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