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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2016, Vol. 17 Issue (9): 692-702    DOI: 10.1631/jzus.B1600017
Articles     
Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide
Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang
Department of Anesthesiology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China; Department of Laboratory Medicine, Bengbu Medical College, Bengbu 233030, China; Department of Anesthesiology, Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China; Department of Parasitology, Bengbu Medical College, Bengbu 233030, China; Department of Immunology, Bengbu Medical College, Bengbu 233030, China
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Abstract  Objective: To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro. Methods: All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of Bax, Bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified. Results: Compared to the NC group, exposure to H2O2 resulted in significant morphological changes, which could be reversed by pretreatment of parecoxib. In addition, H2O2 treatment led to loss of viability (P=0.026) and increased intracellular reactive oxygen species (ROS) levels (P<0.001), and induced apoptosis (P<0.01) in the primary astrocytes relative to the NC group. However, in the parecoxib pretreatment groups, all the above changes reversed significantly (P<0.05) as compared to the H2O2 treatment group, and were nearly unchanged when compared to the NC group. Mechanical investigation showed that dysregulated Bax, Bcl-2, and BDNF could be implicated in these changes. Conclusions: Our results indicated that parecoxib provided a protective effect from oxidative stress induced by exposure to H2O2.

Key words： Parecoxib      Primary astrocyte      Hydrogen peroxide (H2O2)      Brain-derived neurotrophic factor (BDNF)      Bax      Bcl-2     
Received: 16 January 2016      Published: 07 September 2016
CLC:  R965  
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Yun-zhi Ling
Xiao-hong Li
Li Yu
Ye Zhang
Qi-sheng Liang
Xiao-di Yang
Hong-tao Wang
Cite this article:

Yun-zhi Ling, Xiao-hong Li, Li Yu, Ye Zhang, Qi-sheng Liang, Xiao-di Yang, Hong-tao Wang. Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(9): 692-702.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1600017     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2016/V17/I9/692

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