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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2012, Vol. 13 Issue (2): 118-125    DOI: 10.1631/jzus.B1100073
Articles     
Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine
Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China; College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China; Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100080, China
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Abstract  To detect gatifloxacin (GAT) residue in swine urine, an electrochemical immunoassay was established. An indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (ELISA) plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody. Horseradish peroxidase (HRP) conjugated to goat anti-rabbit IgG was used as the enzymatic label. A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator. The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration (IC50) of 8.9 ng/ml was obtained. Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin (3.0%), ciprofloxacin (3.0%), and ofloxacin (1.9%) among commonly used (fluoro)quinolones. In conclusion, the immunoassay system developed in this research can be used as a rapid, powerful and on-site analytical tool to detect GAT residue in foods and food products.

Key words： Gatifloxacin      Drug residue      Antibody      Carbon fiber electrode      Electrochemical immunoassay     
Received: 10 March 2011      Published: 18 January 2012
CLC:  R917  
  O657.1  
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Jian Yi
Ri-mo Xi
Meng Meng
Zhong-qiu Liu
Jin-fang Zhi
Yuan-yang Zhang
Jing Xu
Ya-bin Wang
Jin-ting Liu
Cite this article:

Jian Yi, Meng Meng, Zhong-qiu Liu, Jin-fang Zhi, Yuan-yang Zhang, Jing Xu, Ya-bin Wang, Jin-ting Liu, Ri-mo Xi. Development of an electrochemical immunoassay for detection of gatifloxacin in swine urine. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(2): 118-125.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1100073     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2012/V13/I2/118

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