Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2006, Vol. 7 Issue (2 ): 12-    DOI: 10.1631/jzus.2006.B0154
    
A study of the relationship between expression level of TRF1 protein and telomerase activity in human acute leukemia
Shi Ji-min, Huang He, Chen Qiao-fang, Lin Mao-fang
Department of Hematology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  Objective: To study the expression level of TRF1 (telomeric repeat binding factor 1) protein in human acute leukemia and relationship between expression level of TRF1 protein and telomerase. Methods: A quantitative Western-Blot technique was developed using anti-TRF133-277 monoclonal antibody and GST-TRF1 purity protein as a standard to further determine the expression level of TRF1 protein in total proteins extracted from clinical specimens. Results: Bone marrow tissues of 20 acute leukemia patients were studied, 11 healthy donors’ bone marrows were taken as a control. The expression level of TRF1 protein was significantly higher (P<0.01) in normal bone marrow ((2.217±0.462) µg/µl) than that of acute leukemia patients ((0.754±0.343) µg/µl). But there was no remarkable difference between ALL and ANLL patients ((0.618±0.285) µg/µl vs (0.845±0.359) µg/µl, P>0.05). After chemotherapy, TRF1 expression level of patients with complete remission elevated ((0.772±0.307) µg/µl vs (1.683±0.344) µg/µl, P<0.01), but lower than that of normal ((2.217±0.462) µg/µl, P<0.01). There was no significantly difference after chemotherapy ((0.726±0.411) µg/µl vs (0.895±0.339) µg/µl, P>0.05). TRF1 expression level of patients with complete remission is higher than that of patients without complete remission ((1.683±0.344) µg/µl vs (0.895±0.339) µg/µl, P<0.01). All samples were determined for telomerase activity. It was confirmed that the activity of telomerase in normal bone marrow was lower than that of acute leukemia patients ((0.125±0.078) µg/µl vs (0.765±0.284) µg/µl, P<0.01). There was no significant difference of expression level of TRF1 protein between ALL and ANLL patients ((0.897±0.290) µg/µl vs (0.677±0.268) µg/µl, P>0.05). After chemotherapy, telomerase activity of patients with complete remission decreased ((0.393±0.125) µg/µl), but was still higher than that of normal ((0.125±0.078) µg/µl, P<0.01). Conclusion: The expression level of TRF1 protein has correlativity to the activity of telomerase (P<0.001).

Key words： Acute leukemia (AL)      Human telomeric repeat binding factor protein 1 (TRF1)      Monoclonal antibody      Expression level of TRF1 protein      Telomerase activity     
Received: 29 August 2005     
CLC:  R733.7  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Shi Ji-min
Huang He
Chen Qiao-fang
Lin Mao-fang
Cite this article:

Shi Ji-min, Huang He, Chen Qiao-fang, Lin Mao-fang. A study of the relationship between expression level of TRF1 protein and telomerase activity in human acute leukemia. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(2 ): 12-.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.2006.B0154     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2006/V7/I2 /12

[1] Rui Liu, Ying Liu, Mei-jing Lan, Niusha Taheri, Jing-li Cheng, Yi-rong Guo, Guo-nian Zhu. Evaluation of a water-soluble adjuvant for the development of monoclonal antibodies against small-molecule compounds[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(4): 282-293.
[2] Tossapon Wongtangprasert, Wirongrong Natakuathung, Umaporn Pimpitak, Anumart Buakeaw, Tanapat Palaga, Kittinan Komolpis, Nanthika Khongchareonporn. Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(2): 165-172.
[3] Li-na Han, Shuang He, Yu-tang Wang, Li-ming Yang, Si-yu Liu, Ting Zhang. Advances in monoclonal antibody application in myocarditis[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(8): 676-687.
[4] Jian-xiang Wu, Qiang Wang, Huan Liu, Ya-juan Qian, Yan Xie, Xue-ping Zhou. Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(7): 555-562.
[5] Jun ZHANG, Xin-you XIE, Su-wen YANG, Jin WANG, Chao HE. Nicotinamide N-methyltransferase protein expression in renal cell cancer[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(2): 136-143.
[6] Bin Huang, Yun Yin, Lei Lu, Hai Ding, Lin Wang, Ting Yu, Jia-jin Zhu, Xiao-dong Zheng, Yan-zhen Zhang. Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(10): 812-818.
[7] Jian-xiang WU, Shao-en ZHANG, Xue-ping ZHOU. Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(1): 52-60.
[8] BIAN Chang, ZHAO Kui, TONG Guo-xin, ZHU Yong-liang, CHEN Peng. Immortalization of human umbilical vein endothelial cells with telomerase reverse transcriptase and simian virus 40 large T antigen[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(7): 631-636.