Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (8): 599-607    DOI: 10.1631/jzus.B1001011
Articles     
Evaluation of recombinant endostatin in the treatment of atherosclerotic plaques and neovascularization in rabbits
Wei Mao, Jing Kong, Jin Dai, Zhao-quan Huang, Dong-zhi Wang, Gui-bao Ni, Min-li Chen
Department of Cardiology, Zhejiang Traditional Chinese Medical Hospital, Hangzhou 310006, China, First College of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou 310053, China, Department of Pathology, Zhejiang Traditional Chinese Medical Hospital, Hangzhou 310006, China, Center of Experimental Animal, Zhejiang Chinese Medical University, Hangzhou 310053, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  Objective: Atherosclerotic plaques and neovascularization play an important role in the course of coronary atherosclerosis. This study evaluated the effect of recombinant endostatin on experimental atherosclerotic plaques and neovascularization in rabbits. Methods: Eighteen healthy male rabbits were divided into three groups: control group, atherosclerotic model group, and recombinant endostatin treated group. The atherosclerotic model was established via a high-cholesterol diet after balloon catheter injury. The subject weights, serum total cholesterol, creatine kinase-myocardial band fraction (CKMB), and matrix metalloproteinase-2 (MMP-2) were measured. Six weeks after treatment, the aortic roots were taken for pathological assay. The thickness ratio of the intima to media was measured by hematoxylin and eosin (HE) staining, and the number of neovessels was measured by immunohistochemistry via monoclonal antibody CD31 staining. Results: The weight, plasma total cholesterol, and CKMB were not significantly different between the atherosclerotic model group and the recombinant endostatin treated group, but much higher than those of the control group (P<0.05). The thickness ratio of the intima to media in the recombinant endostatin treated group was distinctly less than that in the atherosclerotic model group (P<0.05). The number of neovessels decreased dramatically (P<0.05) and the content of MMP-2 decreased slightly without statistical difference (P>0.05) in the recombinant endostatin treated group, compared to the atherosclerotic model group. Conclusions: Recombinant endostatin is able to inhibit the growth of neovascularization in the atherosclerotic plaque and the development of plaque.

Key words： Recombinant endostatin      Atherosclerosis      Neovessels     
Received: 21 June 2010      Published: 02 August 2010
CLC:  R54  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Jin Dai
Zhao-quan Huang
Wei Mao
Jing Kong
Dong-zhi Wang
Gui-bao Ni
Min-li Chen
Cite this article:

Wei Mao, Jing Kong, Jin Dai, Zhao-quan Huang, Dong-zhi Wang, Gui-bao Ni, Min-li Chen. Evaluation of recombinant endostatin in the treatment of atherosclerotic plaques and neovascularization in rabbits. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(8): 599-607.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1001011     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I8/599

[1] Sheng-an Su, Hong Ma, Li Shen, Mei-xiang Xiang, Jian-an Wang. Interleukin-17 and acute coronary syndrome[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(8): 664-669.
[2] Heng Ge, Qing Zhang, Wei Zhou, Qing He, Zhi-hua Han, Ben He. Efficacy and safety of drug-eluting stent implantation for the treatment of in-stent restenosis occurring within bare-metal stent and drug-eluting stent[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(8): 553-560.
[3] CAI Wei, RUAN Li-ming, WANG Yi-na, CHEN Jun-zhu. Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(8 ): 9-.
[4] YANG Yun-mei, LV Xue-ying, HUANG Wei-dong, XU Zhe-rong, WU Ling-jiao. Study of androgen and atherosclerosis in old-age male[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 9): 15-.