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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (12): 895-904    DOI: 10.1631/jzus.B1000302
Biomedicine     
Improved myocardial perfusion and cardiac function by controlled-release basic fibroblast growth factor using fibrin glue in a canine infarct model
Shao-ping Nie, Xiao Wang, Shi-bin Qiao, Qiu-tang Zeng, Ju-quan Jiang, Xiao-qing Liu, Xiang-ming Zhu, Guo-xiang Cao, Chang-sheng Ma
Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
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Abstract  Objective: Angiogenic therapy is emerging as a potential strategy for the treatment of ischemic heart disease but is limited by a relatively short half-life of growth factors. Fibrin glue (FG) provides a reservoir for controlled-release of growth factors. The aim of this study was to evaluate the effects of basic fibroblast growth factor (bFGF) incorporating FG on angiogenesis and cardiac performance in a canine infarct model. Methods: Acute myocardial infarction was induced by ligation of the left anterior descending coronary artery (LAD). Group I (n=6) underwent ligation of LAD alone. In Group II, transmural channels were created in the infarct area (n=6). In Group III, non-transmural channels were created to locate FG cylinders containing bFGF (n=6). Eight weeks after operation, myocardial perfusion was assessed by single photon emission computed tomography, cardiac function by echocardiography, and vascular development by immunohistochemical staining. Results: Total vascular density and the number of large vessels (internal diameter ≥50 μm) were dramatically higher in Group III than in Groups I and II at eight weeks. Only the controlled-release group exhibited an improvement in regional myocardial perfusion associated with lower defect score. Animals in Group III presented improved cardiac regional systolic and diastolic functions as well as global systolic function in comparison with the other two groups. Conclusions: Enhanced and sustained angiogenic response can be achieved by controlled-release bFGF incorporating FG within transmyocardial laser channels, thus enabling improvement in myocardial perfusion and cardiac function.

Key words： Angiogenesis      Basic fibroblast growth factor      Controlled release      Ischemic heart disease     
Received: 16 August 2010      Published: 09 December 2010
CLC:  R54  
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Chang-sheng Ma
Shi-bin Qiao
Qiu-tang Zeng
Shao-ping Nie
Xiao Wang
Ju-quan Jiang
Xiao-qing Liu
Xiang-ming Zhu
Guo-xiang Cao
Cite this article:

Shao-ping Nie, Xiao Wang, Shi-bin Qiao, Qiu-tang Zeng, Ju-quan Jiang, Xiao-qing Liu, Xiang-ming Zhu, Guo-xiang Cao, Chang-sheng Ma. Improved myocardial perfusion and cardiac function by controlled-release basic fibroblast growth factor using fibrin glue in a canine infarct model. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(12): 895-904.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000302     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I12/895

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