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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (12): 905-911    DOI: 10.1631/jzus.B1000119
Biomedicine     
Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway
Fang Liu, Hui Zhong, Jing-yuan Liang, Ping Fu, Zhi-juan Luo, Li Zhou, Rong Gou, Jun Huang
Division of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China
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Abstract  In this paper, we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs). HASMCs were divided into four groups: normal glucose group (NG), osmolality control group (OC), high glucose group (HG, HASMCs culture medium containing 30 mmol/L glucose), and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN). The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot. After induced by 10 mmol/L β-glycerol phosphoric acid, cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1, 2, and 3, and intracellular calcium contents at Days 7 and 14, respectively. High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P<0.05). The expression of Cbfα-1 was partially blocked by Noggin protein (P<0.05), while BMP-2 was not (P>0.05). After being induced by β-glycerol phosphoric acid, high glucose levels increased the ALP activity [(48.63±1.03) vs. (41.42±2.28) U/mg protein, Day 3; P<0.05] and the intracellular calcium content [(2.76±0.09) vs. (1.75±0.07) μmol/mg protein, Day 14; P<0.05] in a time-dependent manner when compared with the NG group, while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs. (47.37±0.97) U/mg protein, Day 3; P>0.05]. These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway, which can be partially blocked by Noggin protein.

Key words： Bone morphogenetic protein (BMP)      Core binding factor alpha-1 (Cbfα-1)      Vascular smooth muscle cell      Noggin protein     
Received: 30 May 2010      Published: 09 December 2010
CLC:  R543  
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Jing-yuan Liang
Ping Fu
Fang Liu
Hui Zhong
Zhi-juan Luo
Li Zhou
Rong Gou
Jun Huang
Cite this article:

Fang Liu, Hui Zhong, Jing-yuan Liang, Ping Fu, Zhi-juan Luo, Li Zhou, Rong Gou, Jun Huang. Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(12): 905-911.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000119     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I12/905

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