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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (6): 391-401    DOI: 10.1631/jzus.B1000056
Biomedicine     
Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir
Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen
State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong, China, Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China, Department of Microbiology, The University of Hong Kong, Hong Kong, China, Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China
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Abstract  A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.

Key words： Leptotrichia hongkongensis      Bacterium      Blood culture      Anaerobic bacteria      Novel species     
Received: 22 February 2010      Published: 02 June 2010
CLC:  Q93  
  R51  
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Articles by authors
Kwok-Yung Yuen
Patrick C. Y. Woo
Samson S. Y. Wong
Jade L. L. Teng
Kit-Wah Leung
Antonio H. Y. Ngan
Dong-qing Zhao
Herman Tse
Susanna K. P. Lau
Cite this article:

Patrick C. Y. Woo, Samson S. Y. Wong, Jade L. L. Teng, Kit-Wah Leung, Antonio H. Y. Ngan, Dong-qing Zhao, Herman Tse, Susanna K. P. Lau, Kwok-Yung Yuen. Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(6): 391-401.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000056     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I6/391

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