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| Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts |
| Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin |
| Kharkiv National University, pl. Svobody 4, Kharkiv 61077, Ukraine; Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv 61015, Ukraine |
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Abstract To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm2, exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm2 produced no effect, and irradiation at the intensities of 10 and 100 µW/cm2 induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.
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Received: 16 February 2010
Published: 05 October 2010
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Cite this article:
Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin. Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(10): 801-805.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000051 OR http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I10/801
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