Please wait a minute...
ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (4): 286-291    DOI: 10.1631/jzus.B0900344
Biotechnology     
Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.
Hao Yin, Xin-qin Shi, Bo Sun, Jing-jing Ye, Zu-an Duan, Xiao-ling Zhou, Wei-zheng Cui, Xiao-feng Wu
Key Laboratory of Agricultural Ecology and Environment, College of Forestry, Shandong Agricultural University, Taian 271018, China, Institute of Sericultural Sciences of Sichuan Province, Nanchong 637000, China, College of Animal Science, Zhejiang University, Hangzhou 310029, China
Download:     PDF (0 KB)     
Export: BibTeX | EndNote (RIS)      

Abstract  1-deoxynojirimycin (1-DNJ) contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.

Key words： Silkworm      1-deoxynojirimycin (1-DNJ)      Accumulation      Bombyx mori     
Received: 30 October 2009      Published: 29 March 2010
CLC:  Q966  
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Hao Yin
Xin-qin Shi
Bo Sun
Jing-jing Ye
Zu-an Duan
Xiao-ling Zhou
Wei-zheng Cui
Xiao-feng Wu
Cite this article:

Hao Yin, Xin-qin Shi, Bo Sun, Jing-jing Ye, Zu-an Duan, Xiao-ling Zhou, Wei-zheng Cui, Xiao-feng Wu. Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(4): 286-291.

URL:

http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0900344     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I4/286

[1] Wei Hu, Ji-hong Chen, Shu-yang Wang, Jing Liu, Yuan Song, Qing-feng Wu, Wen-jian Li. Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(4): 262-270.
[2] Chen Chen, Yang-yang Fan, Xin Wang, Fei Song, Tao Jiang, Ping Qian, Shun-Ming Tang, Xing-Jia Shen. bmo-miR-0001 and bmo-miR-0015 down-regulate expression of Bombyx mori fibroin light chain gene in vitro[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(2): 127-135.
[3] Lei Zhang, Yan-wen Wang, Zhi-qiang Lu. Midgut immune responses induced by bacterial infection in the silkworm, Bombyx mori[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(10): 875-882.
[4] Wei-dong Yang, Yu-yan Wang, Feng-liang Zhao, Zhe-li Ding, Xin-cheng Zhang, Zhi-qiang Zhu, Xiao-e Yang. Variation in copper and zinc tolerance and accumulation in 12 willow clones: implications for phytoextraction[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2014, 15(9): 788-800.
[5] Li-wen Zhang, Jing-feng Huang, Rui-fang Guo, Xin-xing Li, Wen-bo Sun, Xiu-zhen Wang. Spatio-temporal reconstruction of air temperature maps and their application to estimate rice growing season heat accumulation using multi-temporal MODIS data[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(2): 144-161.
[6] Ling-li Lu, Sheng-ke Tian, Xiao-e Yang, Hong-yun Peng, Ting-qiang Li. Improved cadmium uptake and accumulation in the hyperaccumulator Sedum alfredii: the impact of citric acid and tartaric acid[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(2): 106-114.
[7] Chun-fa Liu, Cheng-xian Wu, Muhammad T. Rafiq, Rukhsanda Aziz, Dan-di Hou, Zhe-li Ding, Zi-wen Lin, Lin-jun Lou, Yuan-yuan Feng, Ting-qiang Li, Xiao-e Yang. Accumulation of mercury in rice grain and cabbage grown on representative Chinese soils[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(12): 1144-1151.
[8] Xing-wei Xiang, Rui Yang, Lin Chen, Xiao-long Hu, Shao-fang Yu, Cui-ping Cao, Xiao-feng Wu. Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2012, 13(2): 111-117.
[9] Yong Huang, Quan Zou, Sheng-peng Wang, Shun-ming Tang, Guo-zheng Zhang, Xing-jia Shen. Construction and detection of expression vectors of microRNA-9a in BmN cells[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2011, 12(7): 527-533.
[10] Na Wang, Cai-ying Jiang, Ming-xing Jiang, Chuan-xi Zhang, Jia-an Cheng. Using chimeric piggyBac transposase to achieve directed interplasmid transposition in silkworm Bombyx mori and fruit fly Drosophila cells[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(9): 728-734.
[11] Yuan-zhi SHI, Jian-yun RUAN, Li-feng MA, Wen-yan HAN, Fang WANG. Accumulation and distribution of arsenic and cadmium by tea plants[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(3): 265-270.
[12] XIE Wen-xia, WANG Guang-huo, ZHANG Qi-chun, GUO Hai-chao. Effects of nitrogen fertilization strategies on nitrogen use efficiency in physiology, recovery, and agronomy and redistribution of dry matter accumulation and nitrogen accumulation in two typical rice cultivars in Zhejiang, China[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(3): 208-216.
[13] ZHANG Hang-jun, ZHANG Jian-ying, HONG Ye, CHEN Ying-xu. Evaluation of organ distribution of microcystins in the freshwater phytoplanktivorous fish Hypophthalmichthys molitrix[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2007, 8(2): 116-120.
[14] WERNER Wilfried, TRIMBORN Manfred, PIHL Uwe. Prediction of the P-leaching potential of arable soils in areas with high livestock densities[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(7 ): 2-.
[15] Rao C.G.P., Seshagiri S.V., Ramesh C., Ibrahim Basha K., Nagaraju H., Chandrashekaraiah. Evaluation of genetic potential of the polyvoltine silkworm (Bombyx mori L.) germplasm and identification of parents for breeding programme[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(3 ): 8-.